Sandbox Reserved 1101: Difference between revisions
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== Monomer structure of the spidroin NTD domain == | == Monomer structure of the spidroin NTD domain == | ||
One monomer of NTD (N-Terminal Domain) is | One monomer of NTD (N-Terminal Domain) is composed of 5 α-helices (H1 to H5). There is also a chain Z composed of 3 amino acids (Ser, Tyr, Gly), but it role is not well established yet. | ||
In each subunit, the orientation of helices 2, 3 and 5 is different from the orientation of helices 1 and 4. Indeed, helices 1 and 4 form the rigid body of the NTD domain, while helices 2, 3 and 5 are involved in intermolecular contacts, so they play an important role in the dimerization process. | In each subunit, the orientation of helices 2, 3 and 5 is different from the orientation of helices 1 and 4. Indeed, helices 1 and 4 form the rigid body of the NTD domain, while helices 2, 3 and 5 are involved in intermolecular contacts, so they play an important role in the dimerization process. | ||
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The charged residues (the acidic and basic ones) are responsible for creating a dipole moment, which therefore implies a non-uniform charge arrangement within the subunits. This is important for the dimerization process, that is why they are highly conserved residues. | The charged residues (the acidic and basic ones) are responsible for creating a dipole moment, which therefore implies a non-uniform charge arrangement within the subunits. This is important for the dimerization process, that is why they are highly conserved residues. | ||
Compared with spidroin of other species of spider, the 2 subunits (A and B) of the NTD | Compared with spidroin of other species of spider, the 2 subunits (A and B) of the dimerized NTD of the spidroin produced by ''N. Clavipes'' are slightly different, due to a different helices arrangement. So they do not completely overlap. This allows the creation of new intermolecular contact networks. | ||
