Sandbox Reserved 110: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
==Crystal structure of MraY bound to carbacaprazamycin== | ==Crystal structure of MraY bound to carbacaprazamycin== | ||
<StructureSection load='6OYH' size='340' side='right' caption='Aricept bound to AChE' scene=''> | <StructureSection load='6OYH' size='340' side='right' caption='Aricept bound to AChE' scene=''> | ||
== Introduction == | |||
'''MraY''', called also '''phospho-N-acetylmuramoyl-pentapeptide-transferase''' or '''UDP-MurNAc-pentapeptide phosphotransferase''', is an integral membrane enzyme involved in peptidoglycan biosynthesis <ref name="one">PMID:29778697</ref> <ref name="two">PMID:27511599</ref>.MraY is encoded by the mraY gene and belongs to a subfamily of the polyprenyl-phosphate N-acetyl hexosamine 1-phosphate transferase (PNPT) superfamily <ref name="three">PMID:23990562</ref>. MRAY is a promising candidate for the development of new antibiotics. In fact, it is the target of five classes of natural nucleoside inhibitors with potent antibacterial activity <ref name="two"/> <ref name="four">PMID:29438582</ref>. | |||
The structure presented in this page correspond to the MraY protein isolated from ''Aquifex aeolicus'' (strain VF5) in complex with carbacaprazamycin | |||
<scene name='61/612822/Aricept_docks_on_tcache/1'>Aricept docked onto AChE</scene> | <scene name='61/612822/Aricept_docks_on_tcache/1'>Aricept docked onto AChE</scene> | ||
== Function == | == Function == | ||
MraY is a critical enzyme in peptidoglycan biosynthesis. Peptidoglycan is an essential component of the cell wall of Gramnegative and Gram-positive bacteria <ref name="five">DOI:10.1039/b816215h</ref>. The cell wall provides bacteria a structural support and protection. In particular, it allows bacteria to maintain their cell shape at different osmotic pressures <ref name="six">PMID:26370936</ref>. Peptidoglycan is a cross-linked polymer of carbohydrates an amino acids and due to its biological relevance in Bacteria, it has been a major target for antibiotics <ref name="three"/> <ref name="five"/>. | |||
Peptidoglycan biosynthesis involves three main stages. MraY is responsible for the second stage. First, the peptidoglycan precursor UDP-Nacetylmuramoyl (MurNAc)–pentapeptide is synthesized in the cytosol. Second, this hydrophilic precursor is attached to a lipid carrier, and the complex lipid carrier-precursor is transported, through the membrane, to the periplasm. Third, the peptidoglycan precursors are polymerized to form the cell wall. MraY catalyzes the transfer of phospho-MurNAc-pentapeptide from hydrophilic substrate UDP-MurNAc-pentapeptide to the lipid carier (C55-P) in the presence of a Mg2+ cofactor. The product is the undecaprenyl-pyrophosphoryl-MurNAcpentapeptide, also known as lipid I <ref name="three"/> <ref name="seven">PMID:31266949</ref> <ref name="eight">PMID:18081839</ref>. | |||
== Structure == | == Structure == | ||
== 3D related | == 3D related structures == | ||
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
