Sandbox Reserved 1101: Difference between revisions

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5IZ2 is the '''NTD domain''' of a protein called [https://en.wikipedia.org/wiki/Spidroin_ spidroin]. This protein is a component of the dragline silk. There are several types of spidroin, and those that form the core of the silk are called '''MaSp1''' (Major ampullate Spidroin-1), which are produced by in the major ampullate gland of spiders.

5IZ2 is the '''NTD domain''' of a protein called [https://en.wikipedia.org/wiki/Spidroin_ spidroin]. This protein is a component of the '''dragline silk'''. There are several types of spidroin, and those that form the core of the silk are called '''MaSp1''' (Major ampullate Spidroin-1), which are produced by in the major ampullate gland of spiders.

The NTD domain of these proteins is very important since it plays a major role in the ~~dimerisation~~ of spidroins. Indeed, thanks to the NTD organization, two spidroins can be combined, leading to the production of fibres with exceptional physical qualities.

The NTD domain of these proteins is very important since it plays a major role in the '''dimerization of spidroins'''. Indeed, thanks to the NTD organization, two spidroins can be combined, leading to the production of fibres with exceptional physical qualities.

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In each subunit, the orientation of helices 2, 3 and 5 is different from the orientation of helices 1 and 4. Indeed, helices 1 and 4 form the rigid body of the NTD domain, while helices 2, 3 and 5 are involved in intermolecular contacts, so they play an important role in the dimerization process.

Moreover, at the opposite extremities of each subunits of the monomer there are clusters of acidic residus (Asp36, Asp39, Asp40, Glu79, Asp91) in one part, and clusters of basic residus (Lys54, Arg57, Lys60, Lys64, Lys65) in the other part~~. So, this create a dipole moment~~. In addition to this, the subunits A and B are organized antiparallel, which allows an access to charges poles.

Moreover, at the opposite extremities of each subunits of the monomer there are '''clusters of acidic residus''' (Asp36, Asp39, Asp40, Glu79, Asp91) in one part, and '''clusters of basic residus''' (Lys54, Arg57, Lys60, Lys64, Lys65) in the other part. In addition to this, the subunits A and B are organized antiparallel, which allows an access to charges poles.

The charged residues (the acidic and basic ones) are responsible for creating a dipole moment, which therefore implies a non-uniform charge arrangement within the subunits. This is important for the dimerization process, that is why they are highly conserved residues.

The charged residues (the acidic and basic ones) are responsible for creating a '''dipole moment''', which therefore implies a non-uniform charge arrangement within the subunits. This is important for the dimerization process, that is why they are highly conserved residues.

Compared with spidroin of other species of spider, the 2 subunits (A and B) of the dimerized NTD of the spidroin produced by ''N. Clavipes'' are slightly different, due to a different helices arrangement. So they do not completely overlap. This allows the creation of new intermolecular contact networks. There is also a <scene name='82/829354/Chain_z/1'>chain Z</scene> composed of 3 amino acids (Ser, Tyr, Gly), but it role is not well established yet.

Compared with spidroin of other species of spider, the 2 subunits (A and B) of the dimerized NTD of the spidroin produced by ''N. Clavipes'' are slightly different, due to a '''different helices arrangement'''. So they do not completely overlap. This allows the creation of '''new intermolecular contact networks'''. There is also a <scene name='82/829354/Chain_z/1'>chain Z</scene> composed of 3 amino acids (Ser, Tyr, Gly), but it role is not well established yet.

Sandbox Reserved 1101: Difference between revisions

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@@ Line 5: / Line 5: @@
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-IZ2 is the '''NTD domain''' of a protein called [https://en.wikipedia.org/wiki/Spidroin_ spidroin]. This protein is a component of the dragline silk. There are several types of spidroin, and those that form the core of the silk are called '''MaSp1''' (Major ampullate Spidroin-1), which are produced by in the major ampullate gland of spiders.
+IZ2 is the '''NTD domain''' of a protein called [https://en.wikipedia.org/wiki/Spidroin_ spidroin]. This protein is a component of the '''dragline silk'''. There are several types of spidroin, and those that form the core of the silk are called '''MaSp1''' (Major ampullate Spidroin-1), which are produced by in the major ampullate gland of spiders.
-The NTD domain of these proteins is very important since it plays a major role in the dimerisation of spidroins. Indeed, thanks to the NTD organization, two spidroins can be combined, leading to the production of fibres with exceptional physical qualities.
+The NTD domain of these proteins is very important since it plays a major role in the '''dimerization of spidroins'''. Indeed, thanks to the NTD organization, two spidroins can be combined, leading to the production of fibres with exceptional physical qualities.
@@ Line 24: / Line 24: @@
 In each subunit, the orientation of helices 2, 3 and 5 is different from the orientation of helices 1 and 4. Indeed, helices 1 and 4 form the rigid body of the NTD domain, while helices 2, 3 and 5 are involved in intermolecular contacts, so they play an important role in the dimerization process.
-Moreover, at the opposite extremities of each subunits of the monomer there are clusters of acidic residus (Asp36, Asp39, Asp40, Glu79, Asp91) in one part, and clusters of basic residus (Lys54, Arg57, Lys60, Lys64, Lys65) in the other part. So, this create a dipole moment. In addition to this, the subunits A and B are organized antiparallel, which allows an access to charges poles.
+Moreover, at the opposite extremities of each subunits of the monomer there are '''clusters of acidic residus''' (Asp36, Asp39, Asp40, Glu79, Asp91) in one part, and '''clusters of basic residus''' (Lys54, Arg57, Lys60, Lys64, Lys65) in the other part. In addition to this, the subunits A and B are organized antiparallel, which allows an access to charges poles.
-The charged residues (the acidic and basic ones) are responsible for creating a dipole moment, which therefore implies a non-uniform charge arrangement within the subunits. This is important for the dimerization process, that is why they are highly conserved residues.
+The charged residues (the acidic and basic ones) are responsible for creating a '''dipole moment''', which therefore implies a non-uniform charge arrangement within the subunits. This is important for the dimerization process, that is why they are highly conserved residues.
-Compared with spidroin of other species of spider, the 2 subunits (A and B) of the dimerized NTD of the spidroin produced by ''N. Clavipes'' are slightly different, due to a different helices arrangement. So they do not completely overlap. This allows the creation of new intermolecular contact networks. There is also a <scene name='82/829354/Chain_z/1'>chain Z</scene> composed of 3 amino acids (Ser, Tyr, Gly), but it role is not well established yet.
+Compared with spidroin of other species of spider, the 2 subunits (A and B) of the dimerized NTD of the spidroin produced by ''N. Clavipes'' are slightly different, due to a '''different helices arrangement'''. So they do not completely overlap. This allows the creation of '''new intermolecular contact networks'''. There is also a <scene name='82/829354/Chain_z/1'>chain Z</scene> composed of 3 amino acids (Ser, Tyr, Gly), but it role is not well established yet.