Sandbox Reserved 1091: Difference between revisions
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== Properties == | == Properties == | ||
ASP has its highest activity at pH 7,5 and loses it after heating at 60° for 10 minutes. <ref>Aeromonas sobria serine protease (ASP): a subtilisin family endopeptidase with multiple virulence activities</ref> | ASP has its highest activity at pH 7,5 and loses it after heating at 60° for 10 minutes. <ref>Aeromonas sobria serine protease (ASP): a subtilisin family endopeptidase with multiple virulence activities. Imamura T, Murakami Y, Nitta H. Biol. Chem. 398 1055-1068 (2017)</ref> | ||
Experiments have been done in order to establish the sensitivity of ASP to proteases. In has been found that the ASP protease activity was strongly attenuated by serine protease inhibitors ([http://en.wikipedia.org/wiki/Diisopropyl_fluorophosphate DFP], [http://fr.wikipedia.org/wiki/Fluorure_de_4-(2-aminoéthyl)benzènesulfonyle AEBSF]). Moreover, a soybean [http://en.wikipedia.org/wiki/Trypsin_inhibitor trypsin inhibitor] was shown not to block the proteolytic action of ASP itself but could inhibit the [http://en.m.wikipedia.org/wiki/Vascular_permeability vascular permeability] enhancing activity that follows after injection of ASP into epithelial cells. <ref>Physicochemical and biological properties od an extracellular serine protease od Aeromonas sobria. Ritsuko Yokoyama, Yoshio Fujii et al. Japan (2002)</ref> | Experiments have been done in order to establish the sensitivity of ASP to proteases. In has been found that the ASP protease activity was strongly attenuated by serine protease inhibitors ([http://en.wikipedia.org/wiki/Diisopropyl_fluorophosphate DFP], [http://fr.wikipedia.org/wiki/Fluorure_de_4-(2-aminoéthyl)benzènesulfonyle AEBSF]). Moreover, a soybean [http://en.wikipedia.org/wiki/Trypsin_inhibitor trypsin inhibitor] was shown not to block the proteolytic action of ASP itself but could inhibit the [http://en.m.wikipedia.org/wiki/Vascular_permeability vascular permeability] enhancing activity that follows after injection of ASP into epithelial cells. <ref>Physicochemical and biological properties od an extracellular serine protease od Aeromonas sobria. Ritsuko Yokoyama, Yoshio Fujii et al. Japan (2002)</ref> | ||
