Sandbox Reserved 1102: Difference between revisions

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Zika is a [https://en.wikipedia.org/wiki/Double-stranded_RNA_viruses double-stranded RNA virus]. The [https://en.wikipedia.org/wiki/Viral_replication genome replication] of double-stranded RNA viruses needs the intervention of a [https://en.wikipedia.org/wiki/Helicase helicase].

Protein from Zika is composed of a protease domain at its [https://en.wikipedia.org/wiki/N-terminus N terminus] and a helicase domain at its [https://en.wikipedia.org/wiki/C-terminus C terminus]. The multifunctional helicase belongs to the superfamily 2 (SF2) helicase family. It is this helicase domain that has 5'-triphosphatase activity and that performs the critical and indispensable function of unwinding double-stranded RNA during Zika genome replication. So it is an essential enzyme involved in the cycles of [https://en.wikipedia.org/wiki/ATP_hydrolysis ATP hydrolysis] and behaves as a motor protein in the RNA unwinding. Helicase performs ATP hydrolysis at the 5′ end of RNA to generate energy. ~~The~~ phosphorus atom ~~of the γ-phosphate group~~ of the ATP ~~molecule is attacked by a water~~ molecule; ~~then,~~ the γ-phosphate group is ~~released~~ with ADP. With the use of the energy derived from ATP hydrolysis, helicase translocates along nucleic acid strands and unwinds the double-stranded RNA genome.

Protein from Zika is composed of a protease domain at its [https://en.wikipedia.org/wiki/N-terminus N terminus] and a helicase domain at its [https://en.wikipedia.org/wiki/C-terminus C terminus]. The multifunctional helicase belongs to the superfamily 2 (SF2) helicase family. It is this helicase domain that has 5'-triphosphatase activity and that performs the critical and indispensable function of unwinding double-stranded RNA during Zika genome replication. So it is an essential enzyme involved in the cycles of [https://en.wikipedia.org/wiki/ATP_hydrolysis ATP hydrolysis] and behaves as a motor protein in the RNA unwinding. Helicase performs ATP hydrolysis at the 5′ end of RNA to generate energy. A water molecule attacks one of the phosphorus atom of the ATP molecule; after the phosphate group is freed with ADP. With the use of the energy derived from ATP hydrolysis, helicase translocates along nucleic acid strands and unwinds the double-stranded RNA genome.

[[Image:ATPhydrolysis.jpg | thumb | upright=3,5 | Energy from ATP hydrolysis]]

This step is among the necessary steps when it comes to viral RNA replication. It provides, then, conditions for the polymerization of RNA by an RNA-dependent RNA polymerase and the methylation of RNA by methyltransferase, which is essential for Zika replication. Moreover, it has been noticed that the viral replicative capacity and efficiency can be modified by the ATPase activity and therefore. Moreover, this enzyme can also change the host innate immune response.

Sandbox Reserved 1102: Difference between revisions

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@@ Line 11: / Line 11: @@
 Zika is a [https://en.wikipedia.org/wiki/Double-stranded_RNA_viruses double-stranded RNA virus]. The [https://en.wikipedia.org/wiki/Viral_replication genome replication] of double-stranded RNA viruses needs the intervention of a [https://en.wikipedia.org/wiki/Helicase helicase].
-Protein from Zika is composed of a protease domain at its [https://en.wikipedia.org/wiki/N-terminus N terminus] and a helicase domain at its [https://en.wikipedia.org/wiki/C-terminus C terminus]. The multifunctional helicase belongs to the superfamily 2 (SF2) helicase family. It is this helicase domain that has 5'-triphosphatase activity and that performs the critical and indispensable function of unwinding double-stranded RNA during Zika genome replication. So it is an essential enzyme involved in the cycles of [https://en.wikipedia.org/wiki/ATP_hydrolysis ATP hydrolysis] and behaves as a motor protein in the RNA unwinding. Helicase performs ATP hydrolysis at the 5′ end of RNA to generate energy. The phosphorus atom of the γ-phosphate group of the ATP molecule is attacked by a water molecule; then, the γ-phosphate group is released with ADP. With the use of the energy derived from ATP hydrolysis, helicase translocates along nucleic acid strands and unwinds the double-stranded RNA genome.
+Protein from Zika is composed of a protease domain at its [https://en.wikipedia.org/wiki/N-terminus N terminus] and a helicase domain at its [https://en.wikipedia.org/wiki/C-terminus C terminus]. The multifunctional helicase belongs to the superfamily 2 (SF2) helicase family. It is this helicase domain that has 5'-triphosphatase activity and that performs the critical and indispensable function of unwinding double-stranded RNA during Zika genome replication. So it is an essential enzyme involved in the cycles of [https://en.wikipedia.org/wiki/ATP_hydrolysis ATP hydrolysis] and behaves as a motor protein in the RNA unwinding. Helicase performs ATP hydrolysis at the 5′ end of RNA to generate energy. A water molecule attacks one of the phosphorus atom of the ATP molecule; after the phosphate group is freed with ADP. With the use of the energy derived from ATP hydrolysis, helicase translocates along nucleic acid strands and unwinds the double-stranded RNA genome.
 [[Image:ATPhydrolysis.jpg | thumb | upright=3,5 | Energy from ATP hydrolysis]]
 This step is among the necessary steps when it comes to viral RNA replication. It provides, then, conditions for the polymerization of RNA by an RNA-dependent RNA polymerase and the methylation of RNA by methyltransferase, which is essential for Zika replication. Moreover, it has been noticed that the viral replicative capacity and efficiency can be modified by the ATPase activity and therefore. Moreover, this enzyme can also change the host innate immune response.