Sandbox Reserved 1106: Difference between revisions
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Then, from a nucleotide point of view, hAgo1 shows 6 RNA-specific interactions with ribose 2’OH of let-7 (U1, G2, G4, G5, A7 and A8). There are either direct or water-mediated through the side- or main- chain atoms of hAgo1 like for hAgo2/miR20a. However, there is one interaction presents in hAgo2/miR20a that is missing in hAgo1/let-7 ; it is the one with U6 since this base is slightly modified because of the shift of α7 in the L2 toward the N domain. Furthermore, the first adenine base has a syn conformation around the glycosidic bond and the last two bases are piled in the PAZ domain | Then, from a nucleotide point of view, hAgo1 shows 6 RNA-specific interactions with ribose 2’OH of let-7 (U1, G2, G4, G5, A7 and A8). There are either direct or water-mediated through the side- or main- chain atoms of hAgo1 like for hAgo2/miR20a. However, there is one interaction presents in hAgo2/miR20a that is missing in hAgo1/let-7 ; it is the one with U6 since this base is slightly modified because of the shift of α7 in the L2 toward the N domain. Furthermore, the first adenine base has a syn conformation around the glycosidic bond and the last two bases are piled in the PAZ domain | ||
Finally, one of the most important aspect is the non-5’ phosphorylated strand ; indeed the U1 base of let-7 and the 5’P of hAgo1 are interacted with protein residues of the Mid and PIWI domains which avoid an interaction between them, preventing a phosphorylation which is important for accurate slicing activity. <ref name="source1" | Finally, one of the most important aspect is the non-5’ phosphorylated strand ; indeed the U1 base of let-7 and the 5’P of hAgo1 are interacted with protein residues of the Mid and PIWI domains which avoid an interaction between them, preventing a phosphorylation which is important for accurate slicing activity. <ref name="source1"/> | ||
''Knowing that the nine first 9 nucleotids of let-7 are: 5’AAUAUUAAA3’.'' | ''Knowing that the nine first 9 nucleotids of let-7 are: 5’AAUAUUAAA3’.'' | ||
