4tkj

The 0.87 angstrom X-ray structure of the human heart fatty acid-binding protein complexed with palmitic acid

spinqualitylabelsall models 4tkj, resolution 0.87Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4tkj is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GLP, P6G, PLM Related: 3wbg, 4tjz, 4tkb, 4tkh, 3wvm, 4wbk Gene: FABP3, FABP11, MDGI (HUMAN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [FABPH_HUMAN] FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. Publication Abstract from PubMed Long-chain fatty acids (FAs) with low water solubility require fatty-acid-binding proteins (FABPs) to transport them from cytoplasm to the mitochondria for energy production. However, the precise mechanism by which these proteins recognize the various lengths of simple alkyl chains of FAs with similar high affinity remains unknown. To address this question, we employed a newly developed calorimetric method for comprehensively evaluating the affinity of FAs, sub-Angstrom X-ray crystallography to accurately determine their 3D structure, and energy calculations of the coexisting water molecules using the computer program WaterMap. Our results clearly showed that the heart-type FABP (FABP3) preferentially incorporates a U-shaped FA of C10-C18 using a lipid-compatible water cluster, and excludes longer FAs using a chain-length-limiting water cluster. These mechanisms could help us gain a general understanding of how proteins recognize diverse lipids with different chain lengths. Water-mediated recognition of simple alkyl chains by heart-type Fatty-Acid-binding protein.,Matsuoka S, Sugiyama S, Matsuoka D, Hirose M, Lethu S, Ano H, Hara T, Ichihara O, Kimura SR, Murakami S, Ishida H, Mizohata E, Inoue T, Murata M Angew Chem Int Ed Engl. 2015 Jan 26;54(5):1508-11. doi: 10.1002/anie.201409830., Epub 2014 Dec 9. PMID:25491543 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Fatty acid-binding protein 3D structures

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