User:Nikhil Malvankar/Geobacter pilus structure and function: Difference between revisions
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===Pilus Structure=== | ===Pilus Structure=== | ||
Our electron cryomicroscopic structure of ''Geobacter sulfurreducens'' pili, [[6vk9]], reveals them to be composed of a core of '''<font color='#e87000'>PilA-N</font>''' coated with an outer surface layer of '''<font color='00a0a0'>PilA-C</font>''' (104 amino acids; <scene name='83/834714/Filament/1'>restore initial scene)</scene>. Here is a <scene name='83/834714/Filament/2'>cutaway view</scene> (front half hidden). The C-termini of '''<font color='#e87000'>PilA-N</font>''' <scene name='83/834714/Filament/3'>protrude into sockets</scene> in '''<font color='00a0a0'>PilA-C</font>'''. | Our <scene name='83/834714/Filament/4'>electron cryomicroscopic structure of ''Geobacter sulfurreducens'' pili</scene>, [[6vk9]], reveals them to be composed of a core of '''<font color='#e87000'>PilA-N</font>''' coated with an outer surface layer of '''<font color='00a0a0'>PilA-C</font>''' (104 amino acids; <scene name='83/834714/Filament/1'>restore initial scene)</scene>. Here is a <scene name='83/834714/Filament/2'>cutaway view</scene> (front half hidden). The C-termini of '''<font color='#e87000'>PilA-N</font>''' <scene name='83/834714/Filament/3'>protrude into sockets</scene> in '''<font color='00a0a0'>PilA-C</font>'''. | ||
The PilA-N subunits have extensive hydrophobic contacts with each other, stabilizing the hydrophobic core of the filament. Each PilA-N chain contacts 75 carbon atoms from 11 adjacent PilA-N chains, and also has 4 hydrogen bonds and 4 salt bridges with adjacent PilA-N chains. In contrast, PilA-C subunits have little contact with each other: 14 atoms, which are mostly hydrogen bonded, with one salt bridge. | The PilA-N subunits have extensive hydrophobic contacts with each other, stabilizing the hydrophobic core of the filament. Each PilA-N chain contacts 75 carbon atoms from 11 adjacent PilA-N chains, and also has 4 hydrogen bonds and 4 salt bridges with adjacent PilA-N chains. In contrast, PilA-C subunits have little contact with each other: 14 atoms, which are mostly hydrogen bonded, with one salt bridge. | ||
