6k36: Difference between revisions
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==Crystal structure of BioU from Synechocystis sp.PCC6803 conjugated with DAPA== | |||
<StructureSection load='6k36' size='340' side='right'caption='[[6k36]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6k36]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K36 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K36 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CWF:(8S)-8-azanylnonanoic+acid'>CWF</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k36 OCA], [http://pdbe.org/6k36 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k36 RCSB], [http://www.ebi.ac.uk/pdbsum/6k36 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k36 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In biotin biosynthesis, the conversion of pimeloyl intermediates to biotin is catalyzed by a universal set of four enzymes: BioF, BioA, BioD and BioB. We found that the gene homologous to bioA, the product of which is involved in the conversion of 8-amino-7-oxononanoate (AON) to 7,8-diaminononanoate (DAN), is missing in the genome of the cyanobacterium Synechocystis sp. PCC 6803. We provide structural and biochemical evidence showing that a novel dehydrogenase, BioU, is involved in biotin biosynthesis and functionally replaces BioA. This enzyme catalyzes three reactions: formation of covalent linkage with AON to yield a BioU-DAN conjugate at the epsilon-amino group of Lys124 of BioU using NAD(P)H, carboxylation of the conjugate to form BioU-DAN-carbamic acid, and release of DAN-carbamic acid using NAD(P)(+). In this biosynthetic pathway, BioU is a suicide enzyme that loses the Lys124 amino group after a single round of reaction. | |||
A suicide enzyme catalyzes multiple reactions for biotin biosynthesis in cyanobacteria.,Sakaki K, Ohishi K, Shimizu T, Kobayashi I, Mori N, Matsuda K, Tomita T, Watanabe H, Tanaka K, Kuzuyama T, Nishiyama M Nat Chem Biol. 2020 Feb 10. pii: 10.1038/s41589-019-0461-9. doi:, 10.1038/s41589-019-0461-9. PMID:32042199<ref>PMID:32042199</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 6k36" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Nishiyama, M]] | [[Category: Nishiyama, M]] | ||
[[Category: Sakaki, K]] | [[Category: Sakaki, K]] | ||
[[Category: Tomita, T]] | |||
[[Category: Biotin biosynthesis]] | |||
[[Category: Biou]] | |||
[[Category: Cyanobacteria]] | |||
[[Category: Dehydrogenase]] | |||
[[Category: Oxidoreductase]] | |||
Revision as of 09:13, 26 February 2020
Crystal structure of BioU from Synechocystis sp.PCC6803 conjugated with DAPA
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