4yc0

Crystal structure of ADP-ribosyltransferase Vis in complex with M6 Inhibitor

spinqualitylabelsall models 4yc0, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4yc0 is a 1 chain structure with sequence from "photobacter_splendidum"_(sic)_beijerinck_1900 "photobacter splendidum" (sic) beijerinck 1900. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 5OF Gene: V12B01_18061 ("Photobacter splendidum" (sic) Beijerinck 1900) Activity: NAD(+)--protein-arginine ADP-ribosyltransferase, with EC number 2.4.2.31 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [VIS_VIBSP] A probable mono(ADP-ribosyl)transferase, it may ADP-ribosylate Arg in target protein(s). Upon expression in yeast cells causes cell death. Publication Abstract from PubMed Vis toxin was identified by a bioinformatics strategy as a putative virulence factor produced by Vibrio splendidus with mono-ADP-ribosyltransferase activity. Vis was purified to homogeneity as a 28 kDa single-domain enzyme and was shown to possess NAD(+)-glycohydrolase [KM(NAD(+)) = 276 +/- 12 muM] activity and with an R-S-E-X-E motif; it targets arginine-related compounds [KM(agmatine) = 272 +/- 18 mM]. Mass spectrometry analysis revealed that Vis labels l-arginine with ADP-ribose from the NAD(+) substrate at the amino nitrogen of the guanidinium side chain. Vis is toxic to yeast when expressed in the cytoplasm under control of the CUP1 promotor, and catalytic variants lost the ability to kill the yeast host, indicating that the toxin exerts its lethality through its enzyme activity. Several small molecule inhibitors were identified from a virtual screen, and the most potent compounds were found to inhibit the transferase activity of the enzyme with Ki values ranging from 25 to 134 muM. Inhibitor compound M6 bears the necessary attributes of a solid candidate as a lead compound for therapeutic development. Vis toxin was crystallized, and the structures of the apoenzyme (1.4 A) and the enzyme bound with NAD(+) (1.8 A) and with the M6 inhibitor (1.5 A) were determined. The structures revealed that Vis represents a new subgroup within the mono-ADP-ribosyltransferase toxin family. Characterization of Vis Toxin, a Novel ADP-Ribosyltransferase from Vibrio splendidus.,Ravulapalli R, Lugo MR, Pfoh R, Visschedyk D, Poole A, Fieldhouse RJ, Pai EF, Merrill AR Biochemistry. 2015 Sep 29;54(38):5920-36. doi: 10.1021/acs.biochem.5b00921. Epub , 2015 Sep 17. PMID:26352925 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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