5a7t

Crystal structure of Sulfolobus acidocaldarius Trm10 at 2.4 angstrom resolution.

spinqualitylabelsall models 5a7t, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5a7t is a 1 chain structure with sequence from Atcc 33909. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: PEG NonStd Res: MSE Activity: tRNA (adenine(9)-N(1))-methyltransferase, with EC number 2.1.1.218 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [TRM10_SULAC] Catalyzes the S-adenosyl-L-methionine-dependent formation of N(1)-methyladenine at position 9 (m1A9) in tRNA. Publication Abstract from PubMed Purine nucleosides on position 9 of eukaryal and archaeal tRNAs are frequently modified in vivo by the post-transcriptional addition of a methyl group on their N1 atom. The methyltransferase Trm10 is responsible for this modification in both these domains of life. While certain Trm10 orthologues specifically methylate either guanosine or adenosine at position 9 of tRNA, others have a dual specificity. Until now structural information about this enzyme family was only available for the catalytic SPOUT domain of Trm10 proteins that show specificity toward guanosine. Here, we present the first crystal structure of a full length Trm10 orthologue specific for adenosine, revealing next to the catalytic SPOUT domain also N- and C-terminal domains. This structure hence provides crucial insights in the tRNA binding mechanism of this unique monomeric family of SPOUT methyltransferases. Moreover, structural comparison of this adenosine-specific Trm10 orthologue with guanosine-specific Trm10 orthologues suggests that the N1 methylation of adenosine relies on additional catalytic residues. Structural and functional insights into tRNA binding and adenosine N1-methylation by an archaeal Trm10 homologue.,Van Laer B, Roovers M, Wauters L, Kasprzak JM, Dyzma M, Deyaert E, Kumar Singh R, Feller A, Bujnicki JM, Droogmans L, Versees W Nucleic Acids Res. 2015 Dec 15. pii: gkv1369. PMID:26673726 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also TRNA methyltransferase

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