3j8w

Cryo-EM reconstruction of quasi-HPV16 complex with H263.A2 Fab

Loading... Status: waiting for Mol* initialization Structural highlights 3j8w is a 13 chain structure with sequence from Human papillomavirus type 16 and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3j8v, 3j8z Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Cryo-electron microscopy (cryo-EM) was used to solve the structures of human papillomavirus type 16 (HPV16) complexed with fragments of antibody (Fab) from three different neutralizing monoclonals (mAbs): H16.1A, H16.14J, and H263.A2. The structure-function analysis revealed predominantly monovalent binding of each Fab with capsid interactions that involved multiple loops from symmetry related copies of the major capsid protein. The residues identified in each Fab-virus interface map to a conformational groove on the surface of the capsomer. In addition to the known involvement of the FG and HI loops, the DE loop was also found to constitute the core of each epitope. Surprisingly, the epitope mapping also identified minor contributions by EF and BC loops. Complementary immunological assays included mAb and Fab neutralization. The specific binding characteristics of mAbs correlated with different neutralizing behaviors in pre- and post-attachment neutralization assays. Structural comparison of four different antibodies interacting with human papillomavirus 16 and mechanisms of neutralization.,Guan J, Bywaters SM, Brendle SA, Lee H, Ashley RE, Makhov AM, Conway JF, Christensen ND, Hafenstein S Virology. 2015 May 18;483:253-263. doi: 10.1016/j.virol.2015.04.016. PMID:25996608 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Major capsid protein L1

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