6c21

Capsid protein in the Staphylococcus aureus phage 80alpha mature capsid

Loading... Status: waiting for Mol* initialization Structural highlights 6c21 is a 7 chain structure with sequence from Bacteriophage 80 alpha. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed In the tailed bacteriophages, DNA is packaged into spherical procapsids, leading to expansion into angular, thin-walled mature capsids. In many cases, this maturation is accompanied by cleavage of the major capsid protein (CP) and other capsid-associated proteins, including the scaffolding protein (SP) that serves as a chaperone for the assembly process. Staphylococcus aureus bacteriophage 80alpha is capable of high frequency mobilization of mobile genetic elements called S. aureus pathogenicity islands (SaPIs), such as SaPI1. SaPI1 redirects the assembly pathway of 80alpha to form capsids that are smaller than those normally made by the phage alone. Both CP and SP of 80alpha are N-terminally processed by a host-encoded protease, Prp. We have analyzed phage mutants that express pre-cleaved or uncleavable versions of CP or SP, and show that the N-terminal sequence in SP is absolutely required for assembly, but does not need to be cleaved in order to produce viable capsids. Mutants with pre-cleaved or uncleavable CP display normal viability. We have used cryo-EM to solve the structures of mature capsids from an 80alpha mutant expressing uncleavable CP, and from wildtype SaPI1. Comparisons with structures of 80alpha and SaPI1 procapsids show that capsid maturation involves major conformational changes in CP, consistent with a release of the CP N-arm by SP. The hexamers reorganize during maturation to accommodate the different environments in the 80alpha and SaPI1 capsids. Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80alpha and SaPI1 Capsids.,Kizziah JL, Manning KA, Dearborn AD, Wall EA, Klenow L, Hill RLL, Spilman MS, Stagg SM, Christie GE, Dokland T Viruses. 2017 Dec 16;9(12). pii: v9120384. doi: 10.3390/v9120384. PMID:29258203 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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