6az0

Mitochondrial ATPase Protease YME1

Loading... Status: waiting for Mol* initialization Structural highlights 6az0 is a 7 chain structure with sequence from Baker's yeast and Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ADP, ATP, MG, ZN NonStd Res: UNK Gene: SCRG_02514 (Baker's yeast) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed We present an atomic model of a substrate-bound inner mitochondrial membrane AAA+ quality control protease in yeast, YME1. Our ~3.4-angstrom cryo-electron microscopy structure reveals how the adenosine triphosphatases (ATPases) form a closed spiral staircase encircling an unfolded substrate, directing it toward the flat, symmetric protease ring. Three coexisting nucleotide states allosterically induce distinct positioning of tyrosines in the central channel, resulting in substrate engagement and translocation to the negatively charged proteolytic chamber. This tight coordination by a network of conserved residues defines a sequential, around-the-ring adenosine triphosphate hydrolysis cycle that results in stepwise substrate translocation. A hingelike linker accommodates the large-scale nucleotide-driven motions of the ATPase spiral relative to the planar proteolytic base. The translocation mechanism is likely conserved for other AAA+ ATPases. Structure of the mitochondrial inner membrane AAA+ protease YME1 gives insight into substrate processing.,Puchades C, Rampello AJ, Shin M, Giuliano CJ, Wiseman RL, Glynn SE, Lander GC Science. 2017 Nov 3;358(6363). pii: eaao0464. doi: 10.1126/science.aao0464. PMID:29097521 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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