6roh

Cryo-EM structure of the autoinhibited Drs2p-Cdc50p

Loading... Status: waiting for Mol* initialization Structural highlights 6roh is a 2 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BMA, MG, NAG, PSF NonStd Res: BFD Gene: DRS2, YAL026C, FUN38 (Baker's yeast), CDC50, YCR094W, YCR94W (Baker's yeast) Activity: P-type phospholipid transporter, with EC number 7.6.2.1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [ATC3_YEAST] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Potential). Seems to be involved in ribosome assembly. [CDC50_YEAST] Required for polarized cell growth. Publication Abstract from PubMed Type 4 P-type ATPases (P4-ATPases) are lipid flippases that drive the active transport of phospholipids from exoplasmic or luminal leaflets to cytosolic leaflets of eukaryotic membranes. The molecular architecture of P4-ATPases and the mechanism through which they recognize and transport lipids have remained unknown. Here we describe the cryo-electron microscopy structure of the P4-ATPase Drs2p-Cdc50p, a Saccharomyces cerevisiae lipid flippase that is specific to phosphatidylserine and phosphatidylethanolamine. Drs2p-Cdc50p is autoinhibited by the C-terminal tail of Drs2p, and activated by the lipid phosphatidylinositol-4-phosphate (PtdIns4P or PI4P). We present three structures that represent the complex in an autoinhibited, an intermediate and a fully activated state. The analysis highlights specific features of P4-ATPases and reveals sites of autoinhibition and PI4P-dependent activation. We also observe a putative lipid translocation pathway in this flippase that involves a conserved PISL motif in transmembrane segment 4 and polar residues of transmembrane segments 2 and 5, in particular Lys1018, in the centre of the lipid bilayer. Structure and autoregulation of a P4-ATPase lipid flippase.,Timcenko M, Lyons JA, Januliene D, Ulstrup JJ, Dieudonne T, Montigny C, Ash MR, Karlsen JL, Boesen T, Kuhlbrandt W, Lenoir G, Moeller A, Nissen P Nature. 2019 Jun 26. pii: 10.1038/s41586-019-1344-7. doi:, 10.1038/s41586-019-1344-7. PMID:31243363 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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