6kph: Difference between revisions
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==343 K cryoEM structure of Sso-KARI in complex with Mg2+, NADH and CPD== | |||
<StructureSection load='6kph' size='340' side='right'caption='[[6kph]], [[Resolution|resolution]] 2.41Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6kph]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KPH FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9TY:cyclopropane-1,1-dicarboxylic+acid'>9TY</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6kou|6kou]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kph OCA], [http://pdbe.org/6kph PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kph RCSB], [http://www.ebi.ac.uk/pdbsum/6kph PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kph ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Protein functions are temperature-dependent, but protein structures are usually solved at a single (often low) temperature because of limitations on the conditions of crystal growth or protein vitrification. Here we demonstrate the feasibility of solving cryo-EM structures of proteins vitrified at high temperatures, solve 12 structures of an archaeal ketol-acid reductoisomerase (KARI) vitrified at 4-70 degrees C, and show that structures of both the Mg(2+) form (KARI:2Mg(2+)) and its ternary complex (KARI:2Mg(2+):NADH:inhibitor) are temperature-dependent in correlation with the temperature dependence of enzyme activity. Furthermore, structural analyses led to dissection of the induced-fit mechanism into ligand-induced and temperature-induced effects and to capture of temperature-resolved intermediates of the temperature-induced conformational change. The results also suggest that it is preferable to solve cryo-EM structures of protein complexes at functional temperatures. These studies should greatly expand the landscapes of protein structure-function relationships and enhance the mechanistic analysis of enzymatic functions. | |||
Temperature-Resolved Cryo-EM Uncovers Structural Bases of Temperature-Dependent Enzyme Functions.,Chen CY, Chang YC, Lin BL, Huang CH, Tsai MD J Am Chem Soc. 2019 Dec 26;141(51):19983-19987. doi: 10.1021/jacs.9b10687. Epub, 2019 Dec 16. PMID:31829582<ref>PMID:31829582</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6kph" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Tsai, M | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Chang, Y C]] | |||
[[Category: Chen, C Y]] | |||
[[Category: Huang, C H]] | |||
[[Category: Lin, B L]] | |||
[[Category: Tsai, M D]] | |||
[[Category: Complex]] | |||
[[Category: Isomerase]] | |||
Revision as of 10:06, 27 March 2020
343 K cryoEM structure of Sso-KARI in complex with Mg2+, NADH and CPD
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