6duz

Structure of the periplasmic domains of PrgH and PrgK from the assembled Salmonella type III secretion injectisome needle complex

Loading... Status: waiting for Mol* initialization Structural highlights 6duz is a 48 chain structure with sequence from Salmonella enterica subsp. enterica serovar typhimurium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PRGH_SALTY] Required for invasion of epithelial cells. [PRGK_SALTY] Required for invasion of epithelial cells. Could be involved in protein secretion. Publication Abstract from PubMed The bacterial type III secretion system, or injectisome, is a syringe shaped nanomachine essential for the virulence of many disease causing Gram-negative bacteria. At the core of the injectisome structure is the needle complex, a continuous channel formed by the highly oligomerized inner and outer membrane hollow rings and a polymerized helical needle filament which spans through and projects into the infected host cell. Here we present the near-atomic resolution structure of a needle complex from the prototypical Salmonella Typhimurium SPI-1 type III secretion system, with local masking protocols allowing for model building and refinement of the major membrane spanning components of the needle complex base in addition to an isolated needle filament. This work provides significant insight into injectisome structure and assembly and importantly captures the molecular basis for substrate induced gating in the giant outer membrane secretin portal family. Cryo-EM analysis of the T3S injectisome reveals the structure of the needle and open secretin.,Hu J, Worrall LJ, Hong C, Vuckovic M, Atkinson CE, Caveney N, Yu Z, Strynadka NCJ Nat Commun. 2018 Sep 21;9(1):3840. doi: 10.1038/s41467-018-06298-8. PMID:30242280 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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