Sandbox Reserved 1613: Difference between revisions
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<ref name="Fetsch">PMID:15990223</ref> | <ref name="Fetsch">PMID:15990223</ref> | ||
===Stabilization=== | ===Stabilization=== | ||
In the mid 2010's, upgrades to cryo-electron microscopy and the use of 2 antigen binding fragments <scene name='83/832939/Abcg2_with_bound_5d3-fab/4'>(5D3-Fab)</scene> allowed for high resolution images to finally be developed for ABCG2 transporter protein. With these recent discoveries, the understanding of this protein has greatly increased in the last several years. Before these images, ABCG2 had been unable to be recorded at high resolution as the domains were always moving. 5D3 Fab <scene name='83/832939/Fab_binding_site/ | In the mid 2010's, upgrades to cryo-electron microscopy and the use of 2 antigen binding fragments <scene name='83/832939/Abcg2_with_bound_5d3-fab/4'>(5D3-Fab)</scene> allowed for high resolution images to finally be developed for ABCG2 transporter protein. With these recent discoveries, the understanding of this protein has greatly increased in the last several years. Before these images, ABCG2 had been unable to be recorded at high resolution as the domains were always moving. 5D3 Fab <scene name='83/832939/Fab_binding_site/2'>clamps</scene> the two domains together preventing movement of the transporter from inward to outward facing. | ||
== Relevance == | == Relevance == | ||
