Vm24 Scorpion Toxin: Difference between revisions
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Gurolla G.B et al [https://pubmed-ncbi-nlm-nih-gov.ezproxy.uvm.edu/22540187/?from_single_result=Structure%2C+function%2C+and+chemical+synthesis+of+Vaejovis+mexicanus+peptide+24%3A+a+novel+potent+blocker+of+Kv1.3+potassium+channels+of+human+T+lymphocytes.&expanded_search_query=Structure%2C+function%2C+and+chemical+synthesis+of+Vaejovis+mexicanus+peptide+24%3A+a+novel+potent+blocker+of+Kv1.3+potassium+channels+of+human+T+lymphocytes.] isolated the peptide components of ''Vaejovis mexicanus smithi'' venom using high-performance liquid chromatography. Over 200 components were identified. The structure of Vm24 was determined by solution NMR spectroscopy, and it was sequenced via trypsin digestion. The a synthetic Vm24 gene was created and artificially translated to produce the synthetic Vm24 toxin displayed. | Gurolla G.B et al [https://pubmed-ncbi-nlm-nih-gov.ezproxy.uvm.edu/22540187/?from_single_result=Structure%2C+function%2C+and+chemical+synthesis+of+Vaejovis+mexicanus+peptide+24%3A+a+novel+potent+blocker+of+Kv1.3+potassium+channels+of+human+T+lymphocytes.&expanded_search_query=Structure%2C+function%2C+and+chemical+synthesis+of+Vaejovis+mexicanus+peptide+24%3A+a+novel+potent+blocker+of+Kv1.3+potassium+channels+of+human+T+lymphocytes.] isolated the peptide components of ''Vaejovis mexicanus smithi'' venom using high-performance liquid chromatography. Over 200 components were identified. The structure of Vm24 was determined by solution NMR spectroscopy, and it was sequenced via trypsin digestion. The a synthetic Vm24 gene was created and artificially translated to produce the synthetic Vm24 toxin displayed. | ||
==General Structure== | ==General Structure== | ||
Vm24 is a single chain protein consisting of 36 amino acids. It has a molecular weight of 3,873 daltons. It consists of a three strand anti-parallel <scene name='84/842913/Beta/2'>beta sheet</scene> and one single turn alpha helix | Vm24 is a single chain protein consisting of 36 amino acids. It has a molecular weight of 3,873 daltons. It consists of a three strand anti-parallel <scene name='84/842913/Beta/2'>beta sheet</scene> and one single turn <scene name='84/842913/Aplha/1'>alpha helix</scene> with four disulfide bonds holding the chain together. The disulfides exist between Cys6 and Cys26, Cys12 and Cys31, Cys12 and Cys33, and Cys21 and Cys36 | ||
<scene name='84/842913/Beta/2'>Text To Be Displayed</scene> | <scene name='84/842913/Beta/2'>Text To Be Displayed</scene> | ||
