Vm24 Scorpion Toxin: Difference between revisions
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<Structure load='2k9o' size='350' frame='true' align='right' caption='Vm24 Scorpion Toxin [[2k9o]]' scene='Insert optional scene name here' /> | <Structure load='2k9o' size='350' frame='true' align='right' caption='Vm24 Scorpion Toxin [[2k9o]]' scene='Insert optional scene name here' /> | ||
==Protein Source== | ==Protein Source== | ||
Gurolla G.B et al [https://pubmed-ncbi-nlm-nih-gov.ezproxy.uvm.edu/22540187/?from_single_result=Structure%2C+function%2C+and+chemical+synthesis+of+Vaejovis+mexicanus+peptide+24%3A+a+novel+potent+blocker+of+Kv1.3+potassium+channels+of+human+T+lymphocytes.&expanded_search_query=Structure%2C+function%2C+and+chemical+synthesis+of+Vaejovis+mexicanus+peptide+24%3A+a+novel+potent+blocker+of+Kv1.3+potassium+channels+of+human+T+lymphocytes.] isolated the peptide components of ''Vaejovis mexicanus smithi'' venom using high-performance liquid chromatography. Over 200 components were identified. The structure of Vm24 was determined by solution NMR spectroscopy, and it was sequenced via trypsin digestion. The a synthetic Vm24 gene was created and artificially translated to produce the synthetic Vm24 toxin displayed. | Gurolla G.B et al [https://pubmed-ncbi-nlm-nih-gov.ezproxy.uvm.edu/22540187/?from_single_result=Structure%2C+function%2C+and+chemical+synthesis+of+Vaejovis+mexicanus+peptide+24%3A+a+novel+potent+blocker+of+Kv1.3+potassium+channels+of+human+T+lymphocytes.&expanded_search_query=Structure%2C+function%2C+and+chemical+synthesis+of+Vaejovis+mexicanus+peptide+24%3A+a+novel+potent+blocker+of+Kv1.3+potassium+channels+of+human+T+lymphocytes.] isolated the peptide components of ''Vaejovis mexicanus smithi'' venom using high-performance liquid chromatography. Over 200 components were identified. The structure of Vm24 was determined by solution NMR spectroscopy, and it was sequenced via trypsin digestion. The a synthetic Vm24 gene was created and artificially translated to produce the synthetic Vm24 toxin displayed. The synthetic peptide has the same mass, elution time, and physiological effects as the natural peptide. | ||
==General Structure== | ==General Structure== | ||
Vm24 is a single chain protein consisting of 36 amino acids. It has a molecular weight of 3,873 daltons. It consists of a three strand (S1,S2,S3) anti-parallel <scene name='84/842913/Labeled_beta_strands/1'>beta sheet</scene> and one single turn <scene name='84/842913/Aplha/1'>alpha helix</scene> with <scene name='84/842913/All_disulfides/1'>four disulfide bonds</scene> holding the chain together. | Vm24 is a single chain protein consisting of 36 amino acids. It has a molecular weight of 3,873 daltons. It consists of a three strand (S1,S2,S3) anti-parallel <scene name='84/842913/Labeled_beta_strands/1'>beta sheet</scene> and one single turn <scene name='84/842913/Aplha/1'>alpha helix</scene> with <scene name='84/842913/All_disulfides/1'>four disulfide bonds</scene> holding the chain together. | ||
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==Phylogeny== | ==Phylogeny== | ||
Vm24 is a member of the short scorpion toxin superfamily[https://www.uniprot.org/uniprot/?query=family%3A%22short+scorpion+toxin+superfamily%22&sort=score], potassium channel inhibitor family[https://www.uniprot.org/uniprot/?query=family:%22short+scorpion+toxin+superfamily.+Potassium+channel+inhibitor+family%22&sort=score]. Vm24 appears as a sister clade to the α-KTx subfamilies 6 and 7, and is thus proposed as a novel new subfamily α-KTx 23.1[https://www.uniprot.org/uniprot/?query=family:%22short+scorpion+toxin+superfamily.+Potassium+channel+inhibitor+family.+Alpha-KTx+23+subfamily%22&sort=score]. The alpha-KTx 23 subfamily contains proteins with the CSalpha/beta fold, which comprises one or two short alpha helices connected to anti-parallel beta-sheets stabilized by three or four disulfide bonds. | Vm24 is a member of the short scorpion toxin superfamily[https://www.uniprot.org/uniprot/?query=family%3A%22short+scorpion+toxin+superfamily%22&sort=score], potassium channel inhibitor family[https://www.uniprot.org/uniprot/?query=family:%22short+scorpion+toxin+superfamily.+Potassium+channel+inhibitor+family%22&sort=score]. Vm24 appears as a sister clade to the α-KTx subfamilies 6 and 7, and is thus proposed as a novel new subfamily α-KTx 23.1[https://www.uniprot.org/uniprot/?query=family:%22short+scorpion+toxin+superfamily.+Potassium+channel+inhibitor+family.+Alpha-KTx+23+subfamily%22&sort=score]. The alpha-KTx 23 subfamily contains proteins with the CSalpha/beta fold, which comprises one or two short alpha helices connected to anti-parallel beta-sheets stabilized by three or four disulfide bonds. | ||
==Structural Functionality== | |||
