DNA Polymerase Theta: Difference between revisions
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== Function == | == Function == | ||
DNA polymerase theta (pol theta) is a DNA double strand break repair protein. Double strand breaks can occur as a result of both exogenous (ionizing radiation) and endogenous (reactive oxygen species, replication fork collapse) damage. To repair breaks, polymerase theta employs theta-mediated end-joining (TMEJ), which is a form of alternative non-homologous end-joining (alt-NHEJ). TMEJ begins with pairing of microhomologies in 3' single stranded overhangs that have been exposed through 5' end resectioning at the site of the break. If the microhomologies are internal to the 3' end of the overhanging DNA, the resulting flaps will be removed and a deletion will be introduced. If microhomologies arise from brief templated synthesis with a more distant strand, insertions will | DNA polymerase theta (pol theta) is a DNA double strand break repair protein. Double strand breaks can occur as a result of both exogenous (ionizing radiation) and endogenous (reactive oxygen species, replication fork collapse) damage. To repair breaks, polymerase theta employs theta-mediated end-joining (TMEJ), which is a form of alternative non-homologous end-joining (alt-NHEJ). TMEJ begins with pairing of microhomologies in 3' single stranded overhangs that have been exposed through 5' end resectioning at the site of the break. If the microhomologies are internal to the 3' end of the overhanging DNA, the resulting flaps will be removed and a deletion will be introduced. If microhomologies arise from brief templated synthesis with a more distant strand, insertions will occur. Once microhomologies are aligned, pol theta synthesizes DNA to fill the gaps on either side of the microhomologies. | ||
==Structural Description== | ==Structural Description== | ||
<Structure load='4x0p' size='350' frame='true' align='right' caption='DNA polymerase theta polymerase domain in complex with double-stranded DNA substrate and incoming ddATP' > | <Structure load='4x0p' size='350' frame='true' align='right' caption='DNA polymerase theta polymerase domain in complex with double-stranded DNA substrate and incoming ddATP' > | ||
Pol theta is a member of the A family of polymerases which include | |||
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | ||
Revision as of 18:26, 29 April 2020
Function
DNA polymerase theta (pol theta) is a DNA double strand break repair protein. Double strand breaks can occur as a result of both exogenous (ionizing radiation) and endogenous (reactive oxygen species, replication fork collapse) damage. To repair breaks, polymerase theta employs theta-mediated end-joining (TMEJ), which is a form of alternative non-homologous end-joining (alt-NHEJ). TMEJ begins with pairing of microhomologies in 3' single stranded overhangs that have been exposed through 5' end resectioning at the site of the break. If the microhomologies are internal to the 3' end of the overhanging DNA, the resulting flaps will be removed and a deletion will be introduced. If microhomologies arise from brief templated synthesis with a more distant strand, insertions will occur. Once microhomologies are aligned, pol theta synthesizes DNA to fill the gaps on either side of the microhomologies.
Structural Description
<Structure load='4x0p' size='350' frame='true' align='right' caption='DNA polymerase theta polymerase domain in complex with double-stranded DNA substrate and incoming ddATP' > Pol theta is a member of the A family of polymerases which include
You may include any references to papers as in: the use of JSmol in Proteopedia [1] or to the article describing Jmol [2] to the rescue.
Structural Insights
This is a sample scene created with SAT to color by Group, and another to make a transparent representation of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
</StructureSection>
References
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:https://dx.doi.org/10.1002/ijch.201300024
- ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
