User:Fujr Ibrahim/Sandbox 1: Difference between revisions
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== <h6><span style="color: #808080;"> MLP Extracted from ''Murraya koenigii'' (Heterodimer)</span></h6> == | == <h6><span style="color: #808080;"> MLP Extracted from ''Murraya koenigii'' (Heterodimer)</span></h6> == | ||
This MLP consists of 190 amino acid residues with seven cysteines arranged in three disulfide bridges and has a mass of 21.4kDa. Crystal structure analysis | This MLP consists of 190 amino acid residues with seven cysteines arranged in three disulfide bridges and has a mass of 21.4kDa. Crystal structure analysis shows that the protein is composed of antiparallel beta-strands, loops that connect the beta-strands, and 4 helix turns. Several disulfide bridges that hold the monomers together in a manner similar to that between monomers in miraculin. | ||
<scene name='83/839322/Secondary_structure_3iir/1'>Click here for secondary structure (<span style="color: FF00FF;"> alpha helix </span> and <span style="color: FFFF00;"> beta strand </span> )</scene> | |||
<scene name='83/839322/Cys_res_in_3iir/2'>'Click here to view cys residues'</scene> | |||
<scene name='83/839322/His_residues_3iir/1'>'Click here to view his residues' </scene> | |||
<StructureSection load='3IIR' size='340' side='right' caption='Miraculin-Like Protein Extracted from ''Murraya koenigii''' scene='' /> | <StructureSection load='3IIR' size='340' side='right' caption='Miraculin-Like Protein Extracted from ''Murraya koenigii''' scene='' /> | ||
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== <h6><span style="color: #808080;"> MMLP Extracted from Vitis vinifera (monomer) </span></h6> == | == <h6><span style="color: #808080;"> MMLP Extracted from Vitis vinifera (monomer) </span></h6> == | ||
Another MLP that will be used for protein visualization is that extracted from Vitis vinifera. MLP from grape (Vitis vinifera) exhibits significant homology to miraculin (61% identity). This colorless protein has not been found to display any taste-modifying properties <ref>https://doi.org/10.1016/j.bbapap.2018.08.009 </ref>. Analysis of this MLP’s crystal structure | Another MLP that will be used for protein visualization is that extracted from Vitis vinifera. MLP from grape (Vitis vinifera) exhibits significant homology to miraculin (61% identity). This colorless protein has not been found to display any taste-modifying properties <ref>https://doi.org/10.1016/j.bbapap.2018.08.009 </ref>. Analysis of this MLP’s crystal structure shows the presence of sulfide bridges in pairs almost parallel to another --suggesting binding properties--, a helix turn between loops and numerous antiparallel beta-sheets. | ||
The overall structure of the two MLPs discussed and miraculin’s predicted model appears to be very similar. All three seem to consist of very loosely packed loops, beta-sheets, and disulfide bridges. | The overall structure of the two MLPs discussed and miraculin’s predicted model appears to be very similar. All three seem to consist of very loosely packed loops, beta-sheets, and disulfide bridges. | ||
<scene name='83/839322/Secondary_structure_5yh4/1'>'(<span style="color: FF00FF;"> alpha helix </span> and <span style="color: FFFF00;"> beta strand </span> )'</scene> | |||
<scene name='83/839322/His_residues_5yh4/1'>'Click here to view his residues'</scene> | |||
cys residues: <scene name='83/839322/Cys_residues_5yh4/2'>Cys residues</scene> | |||
<StructureSection load='5YH4' size='340' side='right' caption='Structure of MLP Extracted from ''Vitis vinifera'' (monomer)' scene='' /> | <StructureSection load='5YH4' size='340' side='right' caption='Structure of MLP Extracted from ''Vitis vinifera'' (monomer)' scene='' /> | ||