5j37: Difference between revisions

Revision as of 07:27, 10 June 2020

Crystal structure of 60-mer BFDV Capsid Protein in complex with single stranded DNA

Structural highlights

5j37 is a 10 chain structure with sequence from Bfdv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	PO4
Related:	5j36, 5j09
Gene:	Cap (BFDV)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The assembly and regulation of viral capsid proteins into highly ordered macromolecular complexes is essential for viral replication. Here, we utilize crystal structures of the capsid protein from the smallest and simplest known viruses capable of autonomously replicating in animal cells, circoviruses, to establish structural and mechanistic insights into capsid morphogenesis and regulation. The beak and feather disease virus, like many circoviruses, encode only two genes: a capsid protein and a replication initiation protein. The capsid protein forms distinct macromolecular assemblies during replication and here we elucidate these structures at high resolution, showing that these complexes reverse the exposure of the N-terminal arginine rich domain responsible for DNA binding and nuclear localization. We show that assembly of these complexes is regulated by single-stranded DNA (ssDNA), and provide a structural basis of capsid assembly around single-stranded DNA, highlighting novel binding interfaces distinct from the highly positively charged N-terminal ARM domain.

Structural insights into the assembly and regulation of distinct viral capsid complexes.,Sarker S, Terron MC, Khandokar Y, Aragao D, Hardy JM, Radjainia M, Jimenez-Zaragoza M, de Pablo PJ, Coulibaly F, Luque D, Raidal SR, Forwood JK Nat Commun. 2016 Oct 4;7:13014. doi: 10.1038/ncomms13014. PMID:27698405^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sarker S, Terron MC, Khandokar Y, Aragao D, Hardy JM, Radjainia M, Jimenez-Zaragoza M, de Pablo PJ, Coulibaly F, Luque D, Raidal SR, Forwood JK. Structural insights into the assembly and regulation of distinct viral capsid complexes. Nat Commun. 2016 Oct 4;7:13014. doi: 10.1038/ncomms13014. PMID:27698405 doi:https://dx.doi.org/10.1038/ncomms13014

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OCA

[1] Sarker S, Terron MC, Khandokar Y, Aragao D, Hardy JM, Radjainia M, Jimenez-Zaragoza M, de Pablo PJ, Coulibaly F, Luque D, Raidal SR, Forwood JK. Structural insights into the assembly and regulation of distinct viral capsid complexes. Nat Commun. 2016 Oct 4;7:13014. doi: 10.1038/ncomms13014. PMID:27698405 doi:https://dx.doi.org/10.1038/ncomms13014

[1]

@@ Line 1: / Line 1: @@
 ==Crystal structure of 60-mer BFDV Capsid Protein in complex with single stranded DNA==
-<StructureSection load='5j37' size='340' side='right' caption='[[5j37]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='5j37' size='340' side='right'caption='[[5j37]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5j37]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J37 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J37 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5j37]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Bfdv Bfdv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J37 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5J37 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5j36|5j36]], [[5j09|5j09]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j37 OCA], [http://pdbe.org/5j37 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j37 RCSB], [http://www.ebi.ac.uk/pdbsum/5j37 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j37 ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=77856 BFDV])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5j37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j37 OCA], [http://pdbe.org/5j37 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j37 RCSB], [http://www.ebi.ac.uk/pdbsum/5j37 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j37 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Bfdv]]
+[[Category: Large Structures]]
 [[Category: Aragao, D]]
 [[Category: Forwood, J K]]

5j37: Difference between revisions

Revision as of 07:27, 10 June 2020

Crystal structure of 60-mer BFDV Capsid Protein in complex with single stranded DNA

Structural highlights

Publication Abstract from PubMed

References

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