6klr: Difference between revisions
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==Crystal structure of human WIPI3 in complex with the WIR-peptide from ATG2A== | ==Crystal structure of human WIPI3 in complex with the WIR-peptide from ATG2A== | ||
<StructureSection load='6klr' size='340' side='right'caption='[[6klr]]' scene=''> | <StructureSection load='6klr' size='340' side='right'caption='[[6klr]], [[Resolution|resolution]] 2.21Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KLR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KLR FirstGlance]. <br> | <table><tr><td colspan='2'>[[6klr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KLR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KLR FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6klr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6klr OCA], [http://pdbe.org/6klr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6klr RCSB], [http://www.ebi.ac.uk/pdbsum/6klr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6klr ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">WDR45B, WDR45L, WIPI3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6klr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6klr OCA], [http://pdbe.org/6klr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6klr RCSB], [http://www.ebi.ac.uk/pdbsum/6klr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6klr ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/ATG2A_HUMAN ATG2A_HUMAN]] Involved in autophagosome assembly, regulating the size of nascent autophagosomes (PubMed:28561066). Also regulates lipid droplets morphology and distribution within the cell (PubMed:22219374, PubMed:28561066).<ref>PMID:22219374</ref> <ref>PMID:28561066</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
WIPI proteins (WIPI1-4) are mammalian PROPPIN family phosphoinositide effectors essential for autophagosome biogenesis. In addition to phosphoinositides, WIPI proteins can recognize a linear WIPI-interacting-region (WIR)-motif, but the underlying mechanism is poorly understood. Here, we determine the structure of WIPI3 in complex with the WIR-peptide from ATG2A. Unexpectedly, the WIR-peptide entwines around the WIPI3 seven-bladed beta-propeller and binds to three sites in blades 1-3. The N-terminal part of the WIR-peptide forms a short strand that augments the periphery of blade 2, the middle segment anchors into an inter-blade hydrophobic pocket between blades 2-3, and the C-terminal aromatic tail wedges into another tailored pocket between blades 1-2. Mutations in three peptide-binding sites disrupt the interactions between WIPI3/4 and ATG2A and impair the ATG2A-mediated autophagic process. Thus, WIPI proteins recognize the WIR-motif by multi-sites in multi-blades and this multi-site-mediated peptide-recognition mechanism could be applicable to other PROPPIN proteins. | |||
Multi-site-mediated entwining of the linear WIR-motif around WIPI beta-propellers for autophagy.,Ren J, Liang R, Wang W, Zhang D, Yu L, Feng W Nat Commun. 2020 Jun 1;11(1):2702. doi: 10.1038/s41467-020-16523-y. PMID:32483132<ref>PMID:32483132</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6klr" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Human]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Feng W]] | [[Category: Feng, W]] | ||
[[Category: Liang | [[Category: Liang, R B]] | ||
[[Category: Ren | [[Category: Ren, J Q]] | ||
[[Category: Lipid binding protein]] | |||
[[Category: Membrane bound protein]] | |||
Revision as of 10:33, 17 June 2020
Crystal structure of human WIPI3 in complex with the WIR-peptide from ATG2A
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