6xdc: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "6xdc" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
==Cryo-EM structure of SARS-CoV-2 ORF3a== | ==Cryo-EM structure of SARS-CoV-2 ORF3a== | ||
<StructureSection load='6xdc' size='340' side='right'caption='[[6xdc]]' scene=''> | <StructureSection load='6xdc' size='340' side='right'caption='[[6xdc]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XDC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XDC FirstGlance]. <br> | <table><tr><td colspan='2'>[[6xdc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/2019-ncov 2019-ncov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XDC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XDC FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xdc OCA], [http://pdbe.org/6xdc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xdc RCSB], [http://www.ebi.ac.uk/pdbsum/6xdc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xdc ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">3a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2697049 2019-nCoV])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xdc OCA], [http://pdbe.org/6xdc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xdc RCSB], [http://www.ebi.ac.uk/pdbsum/6xdc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xdc ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/AP3A_SARS2 AP3A_SARS2]] Forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. Up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. Induces apoptosis in cell culture. Downregulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination.[UniProtKB:P59632] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
SARS-CoV-2 encodes three putative ion channels: E, 8a, and 3a. In related SARS-CoV-1, 3a is implicated in viral release, inflammasome activation, and cell death and its deletion reduces viral titer and morbidity in animal models, suggesting 3a-targeted therapeutics could treat SARS and COVID-19. However, the structural basis for the function of 3a is unknown. Here, we show that SARS-CoV-2 forms large conductance cation channels and present cryo-EM structures of dimeric and tetrameric SARS-CoV-2 3a in lipid nanodiscs. 3a adopts a novel fold and is captured in a closed or inactivated state. A narrow bifurcated exterior pore precludes conduction and leads to a large polar cavity open to the cytosol. 3a function is conserved in a common variant among circulating SARS-CoV-2 that alters the channel pore. We identify 3a-like proteins in Alpha- and Beta-coronaviruses that infect bats and humans, suggesting therapeutics targeting 3a could treat a range of coronaviral diseases. | |||
Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs.,Kern DM, Sorum B, Hoel CM, Sridharan S, Remis JP, Toso DB, Brohawn SG bioRxiv. 2020 Jun 18. doi: 10.1101/2020.06.17.156554. PMID:32587976<ref>PMID:32587976</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6xdc" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: 2019-ncov]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Brohawn | [[Category: Brohawn, S G]] | ||
[[Category: Hoel | [[Category: Hoel, C M]] | ||
[[Category: Kern | [[Category: Kern, D M]] | ||
[[Category: Coronavirus]] | |||
[[Category: Ion channel]] | |||
[[Category: Sars-cov-2]] | |||
[[Category: Transport protein]] | |||
[[Category: Viroporin]] | |||
