6ouy: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==The crystal structure of the isolate tryptophan synthase alpha-chain from Salmonella enterica serovar typhimurium at 1.60 Angstrom resolution== | ==The crystal structure of the isolate tryptophan synthase alpha-chain from Salmonella enterica serovar typhimurium at 1.60 Angstrom resolution== | ||
<StructureSection load='6ouy' size='340' side='right'caption='[[6ouy]]' scene=''> | <StructureSection load='6ouy' size='340' side='right'caption='[[6ouy]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OUY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OUY FirstGlance]. <br> | <table><tr><td colspan='2'>[[6ouy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OUY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OUY FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ouy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ouy OCA], [http://pdbe.org/6ouy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ouy RCSB], [http://www.ebi.ac.uk/pdbsum/6ouy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ouy ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6oso|6oso]], [[5n2p|5n2p]], [[5kmy|5kmy]], [[3vnd|3vnd]], [[3tha|3tha]], [[2ekc|2ekc]], [[2dzp|2dzp]], [[1v7y|1v7y]], [[1wq5|1wq5]], [[1xc4|1xc4]], [[1xcf|1xcf]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">trpA, STM1727 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ouy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ouy OCA], [http://pdbe.org/6ouy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ouy RCSB], [http://www.ebi.ac.uk/pdbsum/6ouy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ouy ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/TRPA_SALTY TRPA_SALTY]] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Backbone assignments for the isolated alpha-subunit of Salmonella typhimurium tryptophan synthase (TS) are reported based on triple resonance solution-state NMR experiments on a uniformly (2)H,(13)C,(15)N-labeled sample. From the backbone chemical shifts, secondary structure and random coil index order parameters (RCI-S(2)) are predicted. Titration with the 3-indole-D-glycerol 3'-phosphate analog, N-(4'-trifluoromethoxybenzenesulfonyl)-2-aminoethyl phosphate (F9), leads to chemical shift perturbations indicative of conformational changes from which an estimate of the dissociation constant is obtained. Comparisons of the backbone chemical-shifts, RCI-S(2) values, and site-specific relaxation times with and without F9 reveal allosteric changes including modulation in secondary structures and loop rigidity induced upon ligand binding. A comparison is made to the X-ray crystal structure of the alpha-subunit in the full TS alphabetabetaalpha bi-enzyme complex and to two new X-ray crystal structures of the isolated TS alpha-subunit reported in this work. | |||
Backbone assignments and conformational dynamics in the S. typhimurium tryptophan synthase alpha-subunit from solution-state NMR.,Sakhrani VV, Hilario E, Caulkins BG, Hatcher-Skeers ME, Fan L, Dunn MF, Mueller LJ J Biomol NMR. 2020 Jul;74(6-7):341-354. doi: 10.1007/s10858-020-00320-2. Epub, 2020 May 15. PMID:32415580<ref>PMID:32415580</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6ouy" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chang C]] | [[Category: Salty]] | ||
[[Category: Dunn | [[Category: Tryptophan synthase]] | ||
[[Category: Fan L]] | [[Category: Chang, C]] | ||
[[Category: Hilario E]] | [[Category: Dunn, M F]] | ||
[[Category: Mueller L]] | [[Category: Fan, L]] | ||
[[Category: Hilario, E]] | |||
[[Category: Mueller, L]] | |||
[[Category: Alpha-chain]] | |||
[[Category: Lyase]] | |||
[[Category: Recombinant protein]] | |||
[[Category: Wild-type]] | |||
