6x0b: Difference between revisions
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==Crystal Structure of Thioredoxin NaTrxh from Nicotiana alata== | ==Crystal Structure of Thioredoxin NaTrxh from Nicotiana alata== | ||
<StructureSection load='6x0b' size='340' side='right'caption='[[6x0b]]' scene=''> | <StructureSection load='6x0b' size='340' side='right'caption='[[6x0b]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X0B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X0B FirstGlance]. <br> | <table><tr><td colspan='2'>[[6x0b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Indian_tobacco Indian tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X0B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X0B FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x0b OCA], [http://pdbe.org/6x0b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x0b RCSB], [http://www.ebi.ac.uk/pdbsum/6x0b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x0b ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Trxh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4087 Indian tobacco])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x0b OCA], [http://pdbe.org/6x0b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x0b RCSB], [http://www.ebi.ac.uk/pdbsum/6x0b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x0b ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Thioredoxins are regulatory proteins that reduce disulfide bonds on target proteins. NaTrxh, which belongs to the plant thioredoxin family h subgroup 2, interacts and reduces the S-RNase enhancing its ribonuclease activity seven-fold, resulting an essential protein for pollen rejection inNicotiana.Here, the crystal structure of NaTrxh at 1.7 A by X-ray diffraction is reported. NaTrxh conserves the typical fold observed in other thioredoxins from prokaryotes and eukaryotes, but it contains extensions towards both N- and C-termini.The NaTrxh N-terminal extension participates in the reduction of S-RNase, and in the structure reported here, this is orientated towards the reactive site. The interaction between SF11-RNase and the NaTrxh N-terminal was simulated and the short-lived complex observed lasted for a tenth of ns. Moreover, we identified certain amino acids as SF11-RNase-E155 and NaTrxh-M104 as good candidates to contribute to the stability of the complex. Furthermore, we simulated the reduction of the C153-C186 SF11-RNase disulfide bond and observed subtle changes that affect the entire core, which might explain the increase in the ribonuclease activity of S-RNase when it is reduced by NaTrxh. | |||
High resolution crystal structure of NaTrxh from Nicotiana alata and its interaction with the S-RNase.,Maria Daniela TR, Lilian GS, Rogelio RS, Javier Andres JD, Yuridia CZ, Felipe CG J Struct Biol. 2020 Jul 16:107578. doi: 10.1016/j.jsb.2020.107578. PMID:32682729<ref>PMID:32682729</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6x0b" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Indian tobacco]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cruz-Garcia F]] | [[Category: Cruz-Garcia, F]] | ||
[[Category: Gonzalez-Segura L]] | [[Category: Gonzalez-Segura, L]] | ||
[[Category: Torres-Rodriguez | [[Category: Torres-Rodriguez, M D]] | ||
[[Category: Natrxh]] | |||
[[Category: Oxidoreductase]] | |||
[[Category: S-rnase]] | |||
[[Category: Self-incompatibility]] | |||
[[Category: Thioredoxin h]] | |||
Revision as of 05:59, 5 August 2020
Crystal Structure of Thioredoxin NaTrxh from Nicotiana alata
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