5lc8

Crystal Structure of specific mutant from Pseudomonas aeruginosa Lipoxygenase at 1.8A resolution

spinqualitylabelsall models 5lc8, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5lc8 is a 1 chain structure with sequence from "bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FE2, GOL, MG, PEG, ZPE Gene: HMPREF1223_10352 ("Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Secreted LOX from Pseudomonas aeruginosa (PA-LOX) has previously been identified as arachidonic acid 15S-lipoxygenating enzyme. Here we report that the substitution of Ala420Gly in PA-LOX leads to an enzyme variant with pronounced dual specificity favoring arachidonic acid 11R-oxygenation. When compared with other LOX-isoforms the oxygen affinity of wild-type PA-LOX is 1-2 orders of magnitude lower (oxygen Km of 0.4mM) but Ala420Gly exchange improved the oxygen affinity (oxygen Km of 0.2mM). Experiments with stereo-specifically deuterated linoleic acid indicated that the formation of both 13S- and 9R-HpODE involves abstraction of the proS-hydrogen from C11 of the fatty acid backbone. To explore the structural basis for the observed functional changes (altered specificity, improved oxygen affinity) we solved the crystal structure of the Ala420Gly mutant of PA-LOX at 1.8A resolution and compared it with the wild-type enzyme. Modeling of fatty acid alignment at the catalytic center suggested that in the wild-type enzyme dioxygen is directed to C15 of arachidonic acid by a protein tunnel, which interconnects the catalytic center with the protein surface. Ala420Gly exchange redirects intra-enzyme oxygen diffusion by bifurcating this tunnel so that C11 of arachidonic acid also becomes accessible for oxygen insertion. The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.,Kalms J, Banthiya S, Yoga EG, Hamberg M, Holzhutter HG, Kuhn H, Scheerer P Biochim Biophys Acta. 2017 Jan 13. pii: S1388-1981(17)30003-3. doi:, 10.1016/j.bbalip.2017.01.003. PMID:28093240 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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