7bvx: Difference between revisions
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==Crystal structure of C-terminal fragment of pilus adhesin SpaC from Lactobacillus rhamnosus GG-Iodide soaked== | ==Crystal structure of C-terminal fragment of pilus adhesin SpaC from Lactobacillus rhamnosus GG-Iodide soaked== | ||
<StructureSection load='7bvx' size='340' side='right'caption='[[7bvx]]' scene=''> | <StructureSection load='7bvx' size='340' side='right'caption='[[7bvx]], [[Resolution|resolution]] 3.36Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BVX FirstGlance]. <br> | <table><tr><td colspan='2'>[[7bvx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_7469 Atcc 7469]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BVX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BVX FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bvx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvx OCA], [http://pdbe.org/7bvx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bvx RCSB], [http://www.ebi.ac.uk/pdbsum/7bvx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvx ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6m3y|6m3y]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CCE29_04955 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=47715 ATCC 7469])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bvx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bvx OCA], [http://pdbe.org/7bvx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bvx RCSB], [http://www.ebi.ac.uk/pdbsum/7bvx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bvx ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Adhesion to cell surfaces is an essential and early prerequisite for successful host colonization by bacteria, and in most instances involves the specificities of various adhesins. Among bacterial Gram-positives, some genera and species mediate attachment to host cells by using long non-flagellar appendages called sortase-dependent pili. A case in point is the beneficial Lactobacillus rhamnosus GG gut-adapted strain that produces the so-called SpaCBA pilus, a structure noted for its promiscuous binding to intestinal mucus and collagen. Structurally, SpaCBA pili are heteropolymers of three different pilin-protein subunits, each with its own location and function in the pilus: backbone SpaA for length, basal SpaB for anchoring, and tip SpaC for adhesion. Previously, we solved the SpaA tertiary structure by X-ray crystallography and also reported on the crystallization of SpaB and SpaC. Here, we reveal the full-length high-resolution (1.9 A) crystal structure of SpaC, a first for a sortase-dependent pilus-bearing commensal. The SpaC structure, unlike the representative four-domain architecture of other Gram-positive tip pilins, espouses an atypically longer five-domain arrangement that includes N-terminal 'binding' and C-terminal 'stalk' regions of two and three domains, respectively. With the prospect of establishing new mechanistic insights, we provide a structural basis for the multi-substrate binding nature of SpaC, as well as a structural model that reconciles its exclusive localization at the SpaCBA pilus tip. | |||
Crystal structure of lactobacillar SpaC reveals an atypical five-domain pilus tip adhesin: Exposing its substrate-binding and assembly in SpaCBA pili.,Kant A, Palva A, von Ossowski I, Krishnan V J Struct Biol. 2020 Jul 9:107571. doi: 10.1016/j.jsb.2020.107571. PMID:32653644<ref>PMID:32653644</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7bvx" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Atcc 7469]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Kant A]] | [[Category: Kant, A]] | ||
[[Category: Krishnan V]] | [[Category: Krishnan, V]] | ||
[[Category: Palva A]] | [[Category: Palva, A]] | ||
[[Category: | [[Category: Von Ossowaski, I]] | ||
[[Category: Cell adhesion]] | |||
[[Category: Fimbria]] | |||
[[Category: Lactobacillus rhamnosus gg]] | |||
[[Category: Pili]] | |||
[[Category: Pilus adhesin]] | |||
[[Category: Probiotic]] | |||
[[Category: Sortase]] | |||
[[Category: Spac]] | |||
[[Category: Spacba pilus]] | |||
[[Category: Surface protein]] | |||
[[Category: Tip pilin]] | |||
Revision as of 06:43, 19 August 2020
Crystal structure of C-terminal fragment of pilus adhesin SpaC from Lactobacillus rhamnosus GG-Iodide soaked
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