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'''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT''' | '''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFL OCA]. | |||
==Reference== | ==Reference== | ||
Solution structure of a DNA decamer duplex containing the stable 3' T.G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: implications for the highly specific 3' T --> C transition of the (6-4) adduct., Lee JH, Hwang GS, Choi BS, Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6632-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10359763 10359763] | Solution structure of a DNA decamer duplex containing the stable 3' T.G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: implications for the highly specific 3' T --> C transition of the (6-4) adduct., Lee JH, Hwang GS, Choi BS, Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6632-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10359763 10359763] | ||
[[Category: Choi, B S.]] | [[Category: Choi, B S.]] | ||
[[Category: Hwang, G S.]] | [[Category: Hwang, G S.]] | ||
[[Category: Lee, J H.]] | [[Category: Lee, J H.]] | ||
[[Category: | [[Category: Deoxyribonucleic acid]] | ||
[[Category: Dna photoproduct]] | |||
[[Category: | [[Category: Mutagenesis]] | ||
[[Category: | [[Category: Nmr]] | ||
[[Category: | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:41:01 2008'' | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 09:41, 2 May 2008
DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT
Overview
The pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct] is one of the major photoproducts induced by UV irradiation of DNA and occurs at TpT sites. The (6-4) adduct is highly mutagenic and leads most often to a 3' T --> C transition with 85% replicating error frequency [LeClerc, J. E., Borden, A. & Lawrence, C. W. (1991) Proc. Natl. Acad. Sci. USA 88, 9685-9689]. To determine the origin of the specific 3' T --> C transition of the (6-4) adduct, we have used experimental NMR restraints and molecular dynamics to determine the solution structure of a (6-4)-lesion DNA decamer duplex that contains a mismatched base pair between the 3' T residue and an opposed G residue. Normal Watson-Crick-type hydrogen bonding is retained at the 5' T of the lesion site. The O2 carbonyl of the 3' T residue forms hydrogen bonds with the imino and amino protons of the opposed G residue. This potential hydrogen bonding stabilizes the overall helix and restores the highly distorted conformation of the (6-4) adduct to the typical B-form-like DNA structure. This structural feature can explain the marked preference for the insertion of an A residue opposite the 5' T and a G residue opposite the 3' T of the (6-4) lesion during trans-lesion synthesis. Thus these insertions yield the predominant 3' T --> C transition.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure of a DNA decamer duplex containing the stable 3' T.G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: implications for the highly specific 3' T --> C transition of the (6-4) adduct., Lee JH, Hwang GS, Choi BS, Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6632-6. PMID:10359763 Page seeded by OCA on Fri May 2 12:41:01 2008
