6w90: Difference between revisions
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==De novo designed NTF2 fold protein NT-9== | ==De novo designed NTF2 fold protein NT-9== | ||
<StructureSection load='6w90' size='340' side='right'caption='[[6w90]]' scene=''> | <StructureSection load='6w90' size='340' side='right'caption='[[6w90]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W90 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W90 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6w90]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W90 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W90 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w90 OCA], [http://pdbe.org/6w90 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w90 RCSB], [http://www.ebi.ac.uk/pdbsum/6w90 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w90 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w90 OCA], [http://pdbe.org/6w90 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w90 RCSB], [http://www.ebi.ac.uk/pdbsum/6w90 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w90 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Naturally occurring proteins vary the precise geometries of structural elements to create distinct shapes optimal for function. We present a computational design method, loop-helix-loop unit combinatorial sampling (LUCS), that mimics nature's ability to create families of proteins with the same overall fold but precisely tunable geometries. Through near-exhaustive sampling of loop-helix-loop elements, LUCS generates highly diverse geometries encompassing those found in nature but also surpassing known structure space. Biophysical characterization showed that 17 (38%) of 45 tested LUCS designs encompassing two different structural topologies were well folded, including 16 with designed non-native geometries. Four experimentally solved structures closely matched the designs. LUCS greatly expands the designable structure space and offers a new paradigm for designing proteins with tunable geometries that may be customizable for novel functions. | |||
Expanding the space of protein geometries by computational design of de novo fold families.,Pan X, Thompson MC, Zhang Y, Liu L, Fraser JS, Kelly MJS, Kortemme T Science. 2020 Aug 28;369(6507):1132-1136. doi: 10.1126/science.abc0881. PMID:32855341<ref>PMID:32855341</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6w90" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Fraser | [[Category: Synthetic construct sequences]] | ||
[[Category: Kortemme T]] | [[Category: Fraser, J S]] | ||
[[Category: Liu L]] | [[Category: Kortemme, T]] | ||
[[Category: Pan X]] | [[Category: Liu, L]] | ||
[[Category: Thompson | [[Category: Pan, X]] | ||
[[Category: Thompson, M C]] | |||
[[Category: De novo fold family]] | |||
[[Category: De novo protein]] | |||
[[Category: Geometric sampling]] | |||
[[Category: Loop-helix-loop motif]] | |||
[[Category: Protein design]] | |||
Revision as of 09:55, 9 September 2020
De novo designed NTF2 fold protein NT-9
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