6wt5: Difference between revisions
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==Structure of a bacterial STING receptor from Capnocytophaga granulosa== | ==Structure of a bacterial STING receptor from Capnocytophaga granulosa== | ||
<StructureSection load='6wt5' size='340' side='right'caption='[[6wt5]]' scene=''> | <StructureSection load='6wt5' size='340' side='right'caption='[[6wt5]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WT5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WT5 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6wt5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_51502 Atcc 51502]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WT5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WT5 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wt5 OCA], [http://pdbe.org/6wt5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wt5 RCSB], [http://www.ebi.ac.uk/pdbsum/6wt5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wt5 ProSAT]</span></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NCTC12948_02565 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=45242 ATCC 51502])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wt5 OCA], [http://pdbe.org/6wt5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wt5 RCSB], [http://www.ebi.ac.uk/pdbsum/6wt5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wt5 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Stimulator of interferon genes (STING) is a receptor in human cells that senses foreign cyclic dinucleotides released during bacterial infection and endogenous cyclic GMP-AMP signalling during viral infection and antitumour immunity(1-5). STING shares no structural homology with other known signalling proteins(6-9), limiting functional analysis and preventing explanation of the origin of cyclic dinucleotide signalling in mammalian innate immunity. Here we discover functional STING homologues encoded within prokaryotic defence islands and reveal a conserved mechanism of signal activation. Crystal structures of bacterial STING define a minimal homodimeric scaffold that selectively responds to c-di-GMP synthesized by a neighbouring cGAS/DncV-like nucleotidyltransferase (CD-NTase) enzyme. Bacterial STING domains couple cyclic dinucleotide recognition with protein filament formation to drive TIR effector domain oligomerization and rapid NAD(+) cleavage. We reconstruct the evolutionary events following acquisition of STING into metazoan innate immunity and determine the structure of a full-length TIR-STING fusion from the Pacific oyster Crassostrea gigas. Comparative structural analysis demonstrates how metazoan-specific additions to the core STING scaffold enabled a switch from direct effector function to regulation of antiviral transcription. Together, our results explain the mechanism of STING-dependent signalling and reveal conservation of a functional cGAS-STING pathway in prokaryotic bacteriophage defence. | |||
STING cyclic dinucleotide sensing originated in bacteria.,Morehouse BR, Govande AA, Millman A, Keszei AFA, Lowey B, Ofir G, Shao S, Sorek R, Kranzusch PJ Nature. 2020 Sep 2. pii: 10.1038/s41586-020-2719-5. doi:, 10.1038/s41586-020-2719-5. PMID:32877915<ref>PMID:32877915</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6wt5" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Atcc 51502]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Govande | [[Category: Govande, A A]] | ||
[[Category: Keszei | [[Category: Keszei, A F.A]] | ||
[[Category: Kranzusch | [[Category: Kranzusch, P J]] | ||
[[Category: Lowey B]] | [[Category: Lowey, B]] | ||
[[Category: Millman A]] | [[Category: Millman, A]] | ||
[[Category: Morehouse | [[Category: Morehouse, B R]] | ||
[[Category: Ofir G]] | [[Category: Ofir, G]] | ||
[[Category: Shao S]] | [[Category: Shao, S]] | ||
[[Category: Sorek R]] | [[Category: Sorek, R]] | ||
[[Category: Cbass]] | |||
[[Category: Cd-ntase]] | |||
[[Category: Cyclic dinucleotide receptor]] | |||
[[Category: Immune effector]] | |||
[[Category: Immune system]] | |||
