2ksy

Solution nmr structure of sensory rhodopsin II

spinqualitylabelsall models 2ksy, 30 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2ksy is a 1 chain structure with sequence from Atcc 35678. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: RET Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Seven-helix membrane proteins represent a challenge for structural biology. Here we report the first NMR structure determination of a detergent-solubilized seven-helix transmembrane (7TM) protein, the phototaxis receptor sensory rhodopsin II (pSRII) from Natronomonas pharaonis, as a proof of principle. The overall quality of the structure ensemble is good (backbone r.m.s. deviation of 0.48 A) and agrees well with previously determined X-ray structures. Furthermore, measurements in more native-like small phospholipid bicelles indicate that the protein structure is the same as in detergent micelles, suggesting that environment-specific effects are minimal when using mild detergents. We use our case study as a platform to discuss the feasibility of similar solution NMR studies for other 7TM proteins, including members of the family of G protein-coupled receptors. Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy.,Gautier A, Mott HR, Bostock MJ, Kirkpatrick JP, Nietlispach D Nat Struct Mol Biol. 2010 Jun;17(6):768-74. Epub 2010 May 30. PMID:20512150 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Bacteriorhodopsin 3D structures Rhodopsin 3D structures

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