User:Jordan Scott/Sandbox RNA polII: Difference between revisions
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1. <scene name='82/824648/Tfiid-tbp/3'>TFIID</scene> is highly conserved among eukaryotes. It recognizes and binds the TATA box. This is facilitated by a subunit named the TATA-binding protein (TBP). This subunit also causes slight deformations in the helix which may be important for further binding of the PIC units. | 1. <scene name='82/824648/Tfiid-tbp/3'>TFIID</scene> is highly conserved among eukaryotes. It recognizes and binds the TATA box. This is facilitated by a subunit named the TATA-binding protein (TBP). This subunit also causes slight deformations in the helix which may be important for further binding of the PIC units. | ||
2. <scene name='82/824648/Tfiib/3'>TFIIB</scene> is the second to join the PIC. It is thought to be responsible for stabilizing the TBP/DNA complex and insuring proper directionality . If the orientation is | 2. <scene name='82/824648/Tfiib/3'>TFIIB</scene> is the second to join the PIC. It is thought to be responsible for stabilizing the TBP/DNA complex and tethering the TFIID-RNAPII complex to DNA. It is also important in specifying the the TSS and insuring proper directionality . If the orientation is wrong unfavorable interactions form. Mutagenesis studies also suggest that it works as a bridge between TFIID and pol II. | ||
3. <scene name='82/824648/Tfiif/5'>TFIIF</scene> binds directly to RNAP II and escorts it to the promoter while TFIIB helps the complex bind correctly. | 3. <scene name='82/824648/Tfiif/5'>TFIIF</scene> binds directly to RNAP II and escorts it to the promoter while TFIIB helps the complex bind correctly. | ||
4. <scene name='82/824648/Tfiie/4'>TFIIE</scene> and | 4. <scene name='82/824648/Tfiie/4'>TFIIE</scene> and are sequentially recruited which completes the <scene name='82/824648/Pic/3'>PIC</scene>. | ||
5.<scene name='82/824648/Tfiia/3'>TFIIA</scene> is a co-activator that helps regulate PIC assembly. It was initially thought to be essential for activity. It serves as an enhancer and stabilizes the early complexes. It allows for inhibitory effect to be reduced and activators to be more effective. | 5.<scene name='82/824648/Tfiih/3'>TFIIH</scene> | ||
6.<scene name='82/824648/Tfiia/3'>TFIIA</scene> is a co-activator that helps regulate PIC assembly. It was initially thought to be essential for activity. It serves as an enhancer and stabilizes the early complexes. It allows for inhibitory effect to be reduced and activators to be more effective. | |||
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(E)Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms | (E)Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms | ||
(F)The general transcription factors of RNA | |||
polymerase II | |||
Bushnell, D. A.; Westover, K. D.; Davis, R. E.; Kornberg, R. D. Structural Basis of Transcription: An RNA Polymerase II-TFIIB Cocrystal at 4.5 Angstroms. Science. 2004, 303, 983-988 | Bushnell, D. A.; Westover, K. D.; Davis, R. E.; Kornberg, R. D. Structural Basis of Transcription: An RNA Polymerase II-TFIIB Cocrystal at 4.5 Angstroms. Science. 2004, 303, 983-988 | ||
