5vro: Difference between revisions

Revision as of 08:43, 30 September 2020

ABA-mimicking ligand AMF1beta in complex with ABA receptor PYL2 and PP2C HAB1

Structural highlights

5vro is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	A7S, MG
Related:	5vr7, 5vsq, 5vsr, 5vs5, 5vt7
Activity:	Phosphoprotein phosphatase, with EC number 3.1.3.16
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PYL2_ARATH] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA.^[1] ^[2] [P2C16_ARATH] Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, seed germination and inhibition of vegetative growth. Confers enhanced sensitivity to drought.^[3] ^[4] ^[5] ^[6]

Publication Abstract from PubMed

Drought stress is a major threat to crop production, but effective methods to mitigate the adverse effects of drought are not available. Here, we report that adding fluorine atoms in the benzyl ring of the abscisic acid (ABA) receptor agonist AM1 optimizes its binding to ABA receptors by increasing the number of hydrogen bonds between the compound and the surrounding amino acid residues in the receptor ligand-binding pocket. The new chemicals, known as AMFs, have long-lasting effects in promoting stomatal closure and inducing the expression of stress-responsive genes. Application of AMFs or transgenic overexpression of the receptor PYL2 in Arabidopsis and soybean plants confers increased drought resistance. The greatest increase in drought resistance is achieved when AMFs are applied to the PYL2-overexpression transgenic plants. Our results demonstrate that the combining of potent chemicals with transgenic overexpression of an ABA receptor is very effective in helping plants combat drought stress.

Combining chemical and genetic approaches to increase drought resistance in plants.,Cao MJ, Zhang YL, Liu X, Huang H, Zhou XE, Wang WL, Zeng A, Zhao CZ, Si T, Du J, Wu WW, Wang FX, Xu HE, Zhu JK Nat Commun. 2017 Oct 30;8(1):1183. doi: 10.1038/s41467-017-01239-3. PMID:29084945^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Melcher K, Ng LM, Zhou XE, Soon FF, Xu Y, Suino-Powell KM, Park SY, Weiner JJ, Fujii H, Chinnusamy V, Kovach A, Li J, Wang Y, Li J, Peterson FC, Jensen DR, Yong EL, Volkman BF, Cutler SR, Zhu JK, Xu HE. A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Nature. 2009 Dec 3;462(7273):602-8. PMID:19898420 doi:10.1038/nature08613
↑ Yin P, Fan H, Hao Q, Yuan X, Wu D, Pang Y, Yan C, Li W, Wang J, Yan N. Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Nat Struct Mol Biol. 2009 Dec;16(12):1230-6. Epub 2009 Nov 5. PMID:19893533 doi:10.1038/nsmb.1730
↑ Saez A, Apostolova N, Gonzalez-Guzman M, Gonzalez-Garcia MP, Nicolas C, Lorenzo O, Rodriguez PL. Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling. Plant J. 2004 Feb;37(3):354-69. PMID:14731256
↑ Robert N, Merlot S, N'guyen V, Boisson-Dernier A, Schroeder JI. A hypermorphic mutation in the protein phosphatase 2C HAB1 strongly affects ABA signaling in Arabidopsis. FEBS Lett. 2006 Aug 21;580(19):4691-6. Epub 2006 Jul 24. PMID:16876791 doi:10.1016/j.febslet.2006.07.047
↑ Saez A, Robert N, Maktabi MH, Schroeder JI, Serrano R, Rodriguez PL. Enhancement of abscisic acid sensitivity and reduction of water consumption in Arabidopsis by combined inactivation of the protein phosphatases type 2C ABI1 and HAB1. Plant Physiol. 2006 Aug;141(4):1389-99. Epub 2006 Jun 23. PMID:16798945 doi:10.1104/pp.106.081018
↑ Saez A, Rodrigues A, Santiago J, Rubio S, Rodriguez PL. HAB1-SWI3B interaction reveals a link between abscisic acid signaling and putative SWI/SNF chromatin-remodeling complexes in Arabidopsis. Plant Cell. 2008 Nov;20(11):2972-88. doi: 10.1105/tpc.107.056705. Epub 2008 Nov, 25. PMID:19033529 doi:10.1105/tpc.107.056705
↑ Cao MJ, Zhang YL, Liu X, Huang H, Zhou XE, Wang WL, Zeng A, Zhao CZ, Si T, Du J, Wu WW, Wang FX, Xu HE, Zhu JK. Combining chemical and genetic approaches to increase drought resistance in plants. Nat Commun. 2017 Oct 30;8(1):1183. doi: 10.1038/s41467-017-01239-3. PMID:29084945 doi:https://dx.doi.org/10.1038/s41467-017-01239-3

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Melcher K, Ng LM, Zhou XE, Soon FF, Xu Y, Suino-Powell KM, Park SY, Weiner JJ, Fujii H, Chinnusamy V, Kovach A, Li J, Wang Y, Li J, Peterson FC, Jensen DR, Yong EL, Volkman BF, Cutler SR, Zhu JK, Xu HE. A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Nature. 2009 Dec 3;462(7273):602-8. PMID:19898420 doi:10.1038/nature08613

[2] Yin P, Fan H, Hao Q, Yuan X, Wu D, Pang Y, Yan C, Li W, Wang J, Yan N. Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Nat Struct Mol Biol. 2009 Dec;16(12):1230-6. Epub 2009 Nov 5. PMID:19893533 doi:10.1038/nsmb.1730

[3] Saez A, Apostolova N, Gonzalez-Guzman M, Gonzalez-Garcia MP, Nicolas C, Lorenzo O, Rodriguez PL. Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling. Plant J. 2004 Feb;37(3):354-69. PMID:14731256

[4] Robert N, Merlot S, N'guyen V, Boisson-Dernier A, Schroeder JI. A hypermorphic mutation in the protein phosphatase 2C HAB1 strongly affects ABA signaling in Arabidopsis. FEBS Lett. 2006 Aug 21;580(19):4691-6. Epub 2006 Jul 24. PMID:16876791 doi:10.1016/j.febslet.2006.07.047

[5] Saez A, Robert N, Maktabi MH, Schroeder JI, Serrano R, Rodriguez PL. Enhancement of abscisic acid sensitivity and reduction of water consumption in Arabidopsis by combined inactivation of the protein phosphatases type 2C ABI1 and HAB1. Plant Physiol. 2006 Aug;141(4):1389-99. Epub 2006 Jun 23. PMID:16798945 doi:10.1104/pp.106.081018

[6] Saez A, Rodrigues A, Santiago J, Rubio S, Rodriguez PL. HAB1-SWI3B interaction reveals a link between abscisic acid signaling and putative SWI/SNF chromatin-remodeling complexes in Arabidopsis. Plant Cell. 2008 Nov;20(11):2972-88. doi: 10.1105/tpc.107.056705. Epub 2008 Nov, 25. PMID:19033529 doi:10.1105/tpc.107.056705

[7] Cao MJ, Zhang YL, Liu X, Huang H, Zhou XE, Wang WL, Zeng A, Zhao CZ, Si T, Du J, Wu WW, Wang FX, Xu HE, Zhu JK. Combining chemical and genetic approaches to increase drought resistance in plants. Nat Commun. 2017 Oct 30;8(1):1183. doi: 10.1038/s41467-017-01239-3. PMID:29084945 doi:https://dx.doi.org/10.1038/s41467-017-01239-3

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@@ Line 1: / Line 1: @@
 ==ABA-mimicking ligand AMF1beta in complex with ABA receptor PYL2 and PP2C HAB1==
-<StructureSection load='5vro' size='340' side='right' caption='[[5vro]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
+<StructureSection load='5vro' size='340' side='right'caption='[[5vro]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5vro]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VRO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5vro]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VRO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5VRO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A7S:1-(3-fluoro-4-methylphenyl)-N-(2-oxo-1-propyl-1,2,3,4-tetrahydroquinolin-6-yl)methanesulfonamide'>A7S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A7S:1-(3-fluoro-4-methylphenyl)-N-(2-oxo-1-propyl-1,2,3,4-tetrahydroquinolin-6-yl)methanesulfonamide'>A7S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vr7|5vr7]], [[5vsq|5vsq]], [[5vsr|5vsr]], [[5vs5|5vs5]], [[5vt7|5vt7]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vro OCA], [http://pdbe.org/5vro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vro RCSB], [http://www.ebi.ac.uk/pdbsum/5vro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vro ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5vro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vro OCA], [http://pdbe.org/5vro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vro RCSB], [http://www.ebi.ac.uk/pdbsum/5vro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vro ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 20: / Line 20: @@
 </div>
 <div class="pdbe-citations 5vro" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Abscisic acid receptor 3D structures|Abscisic acid receptor 3D structures]]
+*[[Protein Phosphatase 2C|Protein Phosphatase 2C]]
+*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Phosphoprotein phosphatase]]
 [[Category: Cao, M J]]

5vro: Difference between revisions

Revision as of 08:43, 30 September 2020

ABA-mimicking ligand AMF1beta in complex with ABA receptor PYL2 and PP2C HAB1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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Proteopedia Page Contributors and Editors (what is this?)

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