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| <StructureSection load='3mhs' size='340' side='right' caption='Structure of SGF11 (pink) in the SAGA complex with Sus1 (green) , SGF73 (magenta), ubiquitin (yellow), UB8P (grey), glycerol and Zn+2 ions (grey) (PDB entry [[3mhs]])' scene=''> | | <StructureSection load='3mhs' size='340' side='right' caption='Structure of SGF11 (pink) in the SAGA complex with Sus1 (green) , SGF73 (magenta), ubiquitin (yellow), UB8P (grey), glycerol and Zn+2 ions (grey) (PDB entry [[3mhs]])' scene=''> |
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| '''SAGA-associated factor''' (SGF) is part of the coactivator complex SAGA (Spt-Ada-Gcn5-Acetyl transferase) which facilitates transcription of stress-activated genes<ref>PMID:19731963</ref>.<br /> | | '''SAGA-associated factor''' (SGF) is part of the coactivator complex SAGA ('''S'''pt-'''A'''da-'''G'''cn5-'''A'''cetyl transferase) which facilitates transcription of stress-activated genes<ref>PMID:19731963</ref>.<br /> |
| * '''SGF11''' is required for the deubiuqitination of histone H2B and for the maintenance of steady-state H3 methylation level.<br /> | | * '''SGF11''' is required for the deubiuqitination of histone H2B and for the maintenance of steady-state H3 methylation level.<br /> |
| * '''SGF29''' binds methylated lysine of histone H3 and is involved in transcriptional regulation<ref>PMID:23894581</ref>.<br /> | | * '''SGF29''' binds methylated lysine of histone H3 and is involved in transcriptional regulation<ref>PMID:23894581</ref>.<br /> |
Revision as of 09:18, 27 October 2020
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SAGA-associated factor (SGF) is part of the coactivator complex SAGA (Spt-Ada-Gcn5-Acetyl transferase) which facilitates transcription of stress-activated genes[1].
- SGF11 is required for the deubiuqitination of histone H2B and for the maintenance of steady-state H3 methylation level.
- SGF29 binds methylated lysine of histone H3 and is involved in transcriptional regulation[2].
- SGF73 is required for the assembly of RITS complex in yeast[3].
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- ↑ Mischerikow N, Spedale G, Altelaar AF, Timmers HT, Pijnappel WW, Heck AJ. In-depth profiling of post-translational modifications on the related transcription factor complexes TFIID and SAGA. J Proteome Res. 2009 Nov;8(11):5020-30. doi: 10.1021/pr900449e. PMID:19731963 doi:https://dx.doi.org/10.1021/pr900449e
- ↑ Schram AW, Baas R, Jansen PW, Riss A, Tora L, Vermeulen M, Timmers HT. A dual role for SAGA-associated factor 29 (SGF29) in ER stress survival by coordination of both histone H3 acetylation and histone H3 lysine-4 trimethylation. PLoS One. 2013 Jul 23;8(7):e70035. doi: 10.1371/journal.pone.0070035. Print 2013. PMID:23894581 doi:https://dx.doi.org/10.1371/journal.pone.0070035
- ↑ Deng X, Zhou H, Zhang G, Wang W, Mao L, Zhou X, Yu Y, Lu H. Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast. Sci Rep. 2015 Oct 7;5:14707. doi: 10.1038/srep14707. PMID:26443059 doi:https://dx.doi.org/10.1038/srep14707
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3D structures of SAGA-associated factor
Updated on 27-October-2020
{"openlevels":0}
- SGF11
- 2lo2 – ySGF zinc finger domain – yeast – NMR
- 3kik, 3kjl – ySGF Sus1-binding domain + Sus1
- 3mhh, 3m99, 4fip, 4fjc, 4fk5, 4wa6 – yUBP8 + Sus1 +SGF73 - yeast
- 3mhs – yUBP8 + Sus1 +SGF73 + ubiquitin
- 4w4u, 6aqr – ySGF11 + SUS1+ SGF73 + ubiquitin carboxyl terminal hydrolase
- 4zux – yUBP8 + SUS1 + SGF11 +SGF73 + ubiquitin + histone H3.2, H4, H2A, H2B + DNA
- SGF29
- SGF73
- 2lo3 – ySGF zinc finger domain – NMR
References
