1cx5: Difference between revisions
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'''ANTISENSE DNA/RNA HYBRID CONTAINING MODIFIED BACKBONE''' | '''ANTISENSE DNA/RNA HYBRID CONTAINING MODIFIED BACKBONE''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CX5 OCA]. | |||
==Reference== | ==Reference== | ||
NMR structure of an antisense DNA.RNA hybrid duplex containing a 3'-CH(2)N(CH(3))-O-5' or an MMI backbone linker., Yang X, Han X, Cross C, Bare S, Sanghvi Y, Gao X, Biochemistry. 1999 Sep 28;38(39):12586-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504227 10504227] | NMR structure of an antisense DNA.RNA hybrid duplex containing a 3'-CH(2)N(CH(3))-O-5' or an MMI backbone linker., Yang X, Han X, Cross C, Bare S, Sanghvi Y, Gao X, Biochemistry. 1999 Sep 28;38(39):12586-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504227 10504227] | ||
[[Category: Cross, C.]] | [[Category: Cross, C.]] | ||
[[Category: Gao, X.]] | [[Category: Gao, X.]] | ||
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[[Category: Sanghvi, Y.]] | [[Category: Sanghvi, Y.]] | ||
[[Category: Yang, X.]] | [[Category: Yang, X.]] | ||
[[Category: | [[Category: Antisense]] | ||
[[Category: | [[Category: Dna/rna hybrid]] | ||
[[Category: | [[Category: Modified backbone linker]] | ||
[[Category: | [[Category: Nmr]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:12:38 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 10:12, 2 May 2008
ANTISENSE DNA/RNA HYBRID CONTAINING MODIFIED BACKBONE
Overview
The solution structure of an antisense DNA.RNA hybrid duplex, d(CGCGTT-MMI-TTGCGC).r(GCGCAAAACGCG) (designated R4), containing an MMI backbone linker [3'-CH(2)N(CH(3))-O5'], is elucidated. The structural details of the MMI linker, its structural effects on the neighboring residues, and the molecular basis of the MMI effects are examined. The lipophilic N-methyl group of MMI is peripheral to the helix, assuming a conformation that is most stable with regard to the N-O torsion angle. The MMI linker promotes a 3'-endo conformation for the sugar moieties at both 3'- and 5'-adjacent positions and a backbone kink involving distant residues along the 3'-direction. Comparison of R4 with other analogous hybrid duplexes previously studied in this laboratory reveals a new family of low-energy helical conformations that can be accommodated in stable duplexes and a common feature of C3'-modified sugars for adopting a C3'-endo pucker. The results of these studies emphasize the interplay of several factors that govern the formation of stable hybrid duplexes and provide a basis for the understanding of the biological role of the MMI modifications, which are important building blocks for a family of promising chimeric antisense oligonucleotides.
About this Structure
Full crystallographic information is available from OCA.
Reference
NMR structure of an antisense DNA.RNA hybrid duplex containing a 3'-CH(2)N(CH(3))-O-5' or an MMI backbone linker., Yang X, Han X, Cross C, Bare S, Sanghvi Y, Gao X, Biochemistry. 1999 Sep 28;38(39):12586-96. PMID:10504227 Page seeded by OCA on Fri May 2 13:12:38 2008
