6zyk: Difference between revisions
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==Non-heme monooxygenase, ThoJ-Ni complex== | ==Non-heme monooxygenase, ThoJ-Ni complex== | ||
<StructureSection load='6zyk' size='340' side='right'caption='[[6zyk]]' scene=''> | <StructureSection load='6zyk' size='340' side='right'caption='[[6zyk]], [[Resolution|resolution]] 2.55Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZYK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZYK FirstGlance]. <br> | <table><tr><td colspan='2'>[[6zyk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_malaysiense"_ser_et_al._2016 "streptomyces malaysiense" ser et al. 2016]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZYK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZYK FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zyk OCA], [http://pdbe.org/6zyk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zyk RCSB], [http://www.ebi.ac.uk/pdbsum/6zyk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zyk ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VT52_024865 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1428626 "Streptomyces malaysiense" Ser et al. 2016])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zyk OCA], [http://pdbe.org/6zyk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zyk RCSB], [http://www.ebi.ac.uk/pdbsum/6zyk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zyk ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Thioviridamide-like compounds, including thioholgamides, are ribosomally synthesized and post-translationally modified peptide natural products with potent anticancer cell activity and an unprecedented structure. Very little is known about their biosynthesis, and we were intrigued by the beta-hydroxy-N1, N3-dimethylhistidinium moiety found in these compounds. Here we report the construction of a heterologous host capable of producing thioholgamide with a 15-fold increased yield compared to the wild-type strain. A knockout of thoJ, encoding a predicted nonheme monooxygenase, shows that ThoJ is essential for thioholgamide beta-hydroxylation. The crystal structure of ThoJ exhibits a typical mono/dioxygenase fold with conserved key active-site residues. Yet, ThoJ possesses a very large substrate binding pocket that appears suitable to receive a cyclic thioholgamide intermediate for hydroxylation. The improved production of the heterologous host will enable the dissection of the individual biosynthetic steps involved in biosynthesis of this exciting RiPP family. | |||
Non-Heme Monooxygenase ThoJ Catalyzes Thioholgamide beta-Hydroxylation.,Sikandar A, Lopatniuk M, Luzhetskyy A, Koehnke J ACS Chem Biol. 2020 Oct 1. doi: 10.1021/acschembio.0c00637. PMID:32965102<ref>PMID:32965102</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6zyk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Streptomyces malaysiense ser et al. 2016]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Koehnke J]] | [[Category: Koehnke, J]] | ||
[[Category: Sikandar A]] | [[Category: Sikandar, A]] | ||
[[Category: Metal binding protein]] | |||
[[Category: Non-heme monooxygenase]] | |||
