5z95

Structural basis for specific inhibition of highly sensitive ShHTL7 receptor

spinqualitylabelsall models 5z95, resolution 1.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5z95 is a 1 chain structure with sequence from Buchnera hermonthica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EGC, GOL, SO4 Related: 5z82, 5z89, 5z8p Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Striga hermonthica is a root parasitic plant that infests cereals, decimating yields, particularly in sub-Saharan Africa. For germination, Striga seeds require host-released strigolactones that are perceived by the family of HYPOSENSITIVE to LIGHT (ShHTL) receptors. Inhibiting seed germination would thus be a promising approach for combating Striga However, there are currently no strigolactone antagonists that specifically block ShHTLs and do not bind to DWARF14, the homologous strigolactone receptor of the host. Here, we show that the octyl phenol ethoxylate Triton X-100 inhibits S. hermonthica seed germination without affecting host plants. High-resolution X-ray structures reveal that Triton X-100 specifically plugs the catalytic pocket of ShHTL7. ShHTL7-specific inhibition by Triton X-100 demonstrates the dominant role of this particular ShHTL receptor for Striga germination. Our structural analysis provides a rationale for the broad specificity and high sensitivity of ShHTL7, and reveals that strigolactones trigger structural changes in ShHTL7 that are required for downstream signaling. Our findings identify Triton and the related 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]acetic acid as promising lead compounds for the rational design of efficient Striga-specific herbicides. Structural basis for specific inhibition of the highly sensitive ShHTL7 receptor.,Shahul Hameed U, Haider I, Jamil M, Kountche BA, Guo X, Zarban RA, Kim D, Al-Babili S, Arold ST EMBO Rep. 2018 Jul 18. pii: embr.201745619. doi: 10.15252/embr.201745619. PMID:30021834 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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