6eio

Crystal structure of an ice binding protein from an Antarctic Biological Consortium

spinqualitylabelsall models 6eio, resolution 0.84Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6eio is a 1 chain structure with sequence from Uncultivated bacterium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Ice-binding proteins (IBPs) contribute to the survival of many living beings at subzero temperature by controlling the formation and growth of ice crystals. This work investigates the structural basis of the ice-binding properties of EfcIBP, obtained from Antarctic bacteria. EfcIBP is endowed with a unique combination of thermal hysteresis and ice recrystallization inhibition activity. The three-dimensional structure, solved at 0.84 A resolution, shows that EfcIBP belongs to the IBP-1 fold family, and is organized in a right-handed beta-solenoid with a triangular cross-section that forms three protein surfaces, named A, B, and C faces. However, EfcIBP diverges from other IBP-1 fold proteins in relevant structural features including the lack of a 'capping' region on top of the beta-solenoid, and in the sequence and organization of the regions exposed to ice that, in EfcIBP, reveal the presence of threonine-rich ice-binding motifs. Docking experiments and site-directed mutagenesis pinpoint that EfcIBP binds ice crystals not only via its B face, as common to other IBPs, but also via ice-binding sites on the C face. DATABASE: Coordinates and structure factors have been deposited in the Protein Data Bank under accession number 6EIO. Structure of a bacterial ice binding protein with two faces of interaction with ice.,Mangiagalli M, Sarusi G, Kaleda A, Bar Dolev M, Nardone V, Vena VF, Braslavsky I, Lotti M, Nardini M FEBS J. 2018 Mar 13. doi: 10.1111/febs.14434. PMID:29533528 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Antifreeze protein 3D structures

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