6xry: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Intrinsically disordered bacterial polar organizing protein Z, PopZ, interacts with protein binding partners through an N-terminal Molecular Recognition Feature== | ==Intrinsically disordered bacterial polar organizing protein Z, PopZ, interacts with protein binding partners through an N-terminal Molecular Recognition Feature== | ||
<StructureSection load='6xry' size='340' side='right'caption='[[6xry]]' scene=''> | <StructureSection load='6xry' size='340' side='right'caption='[[6xry]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full | <table><tr><td colspan='2'>[[6xry]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cauvc Cauvc]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XRY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XRY FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xry OCA], [http://pdbe.org/6xry PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xry RCSB], [http://www.ebi.ac.uk/pdbsum/6xry PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xry ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">popZ, CC_1319 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=190650 CAUVC])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xry OCA], [http://pdbe.org/6xry PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xry RCSB], [http://www.ebi.ac.uk/pdbsum/6xry PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xry ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The polar organizing protein Z (PopZ) is necessary for the formation of three-dimensional microdomains at the cell poles in Caulobacter crescentus, where it functions as a hub protein that recruits multiple regulatory proteins from the cytoplasm. Although a large portion of the protein is predicted to be natively unstructured, in reconstituted systems PopZ can self-assemble into a macromolecular scaffold that directly binds to at least ten different proteins. Here we report the solution NMR structure of PopZ(Delta134-177), a truncated form of PopZ that does not self-assemble but retains the ability to interact with heterologous proteins. We show that the unbound form of PopZ(Delta134-177) is unstructured in solution, with the exception of a small amphipathic alpha-helix in residues M10-I17, which is included within a highly conserved region near the N-terminus. In applying NMR techniques to map the interactions between PopZ(Delta134-177) and one of its binding partners, RcdA, we find evidence that the alpha-helix and adjoining amino acids extending to position E23 serve as the core of the binding motif. Consistent with this, a point mutation at position I17 severely compromises binding. Our results show that a partially structured Molecular Recognition Feature (MoRF) within an intrinsically disordered domain of PopZ contributes to the assembly of polar microdomains, revealing a structural basis for complex network assembly in Alphaproteobacteria that is analogous to those formed by intrinsically disordered hub proteins in other kingdoms. | |||
Intrinsically disordered bacterial polar organizing protein Z, PopZ, interacts with protein binding partners through an N-terminal Molecular Recognition Feature.,Nordyke CT, Ahmed Y, Puterbaugh RZ, Bowman GR, Varga K J Mol Biol. 2020 Oct 12. pii: S0022-2836(20)30571-4. doi:, 10.1016/j.jmb.2020.09.020. PMID:33058876<ref>PMID:33058876</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6xry" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Cauvc]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Ahmed | [[Category: Ahmed, Y M]] | ||
[[Category: Bowman | [[Category: Bowman, G R]] | ||
[[Category: Nordyke | [[Category: Nordyke, C T]] | ||
[[Category: Puterbaugh | [[Category: Puterbaugh, R Z]] | ||
[[Category: Varga K]] | [[Category: Varga, K]] | ||
[[Category: Hub protein]] | |||
[[Category: Molecular recognition feature]] | |||
[[Category: Morf]] | |||
[[Category: Protein binding]] | |||
