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'''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK''' | '''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDP OCA]. | |||
==Reference== | ==Reference== | ||
Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8525382 8525382] | Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8525382 8525382] | ||
[[Category: Drobny, G P.]] | [[Category: Drobny, G P.]] | ||
[[Category: Hopkins, P B.]] | [[Category: Hopkins, P B.]] | ||
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[[Category: Reid, B R.]] | [[Category: Reid, B R.]] | ||
[[Category: Zhu, L.]] | [[Category: Zhu, L.]] | ||
[[Category: | [[Category: Antitumor drug]] | ||
[[Category: | [[Category: Cis-ddp]] | ||
[[Category: | [[Category: Dna]] | ||
[[Category: | [[Category: Double helix]] | ||
[[Category: | [[Category: Interstrand cross-link]] | ||
[[Category: | [[Category: Nmr]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:44:08 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 10:44, 2 May 2008
SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK
Overview
The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:8525382 Page seeded by OCA on Fri May 2 13:44:08 2008
