1drz: Difference between revisions
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'''U1A SPLICEOSOMAL PROTEIN/HEPATITIS DELTA VIRUS GENOMIC RIBOZYME COMPLEX''' | '''U1A SPLICEOSOMAL PROTEIN/HEPATITIS DELTA VIRUS GENOMIC RIBOZYME COMPLEX''' | ||
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[[Category: Doudna, J A.]] | [[Category: Doudna, J A.]] | ||
[[Category: Zhou, K.]] | [[Category: Zhou, K.]] | ||
[[Category: | [[Category: Catalytic rna]] | ||
[[Category: | [[Category: Hdv]] | ||
[[Category: | [[Category: Ribozyme]] | ||
[[Category: | [[Category: Rna-binding protein]] | ||
[[Category: | [[Category: U1a]] | ||
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Revision as of 11:12, 2 May 2008
U1A SPLICEOSOMAL PROTEIN/HEPATITIS DELTA VIRUS GENOMIC RIBOZYME COMPLEX
Overview
The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.
About this Structure
1DRZ is a Single protein structure of sequence from Hepatitis delta virus and Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:9783582 Page seeded by OCA on Fri May 2 14:12:10 2008
