2ggd

CP4 EPSP synthase Ala100Gly liganded with S3P and Glyphosate

spinqualitylabelsall models 2ggd, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2ggd is a 1 chain structure with sequence from Agrsc. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GPJ, S3P Related: 1g6t, 1g6s, 1q36, 1mi4, 1rf5, 1rf6, 2gg4, 2gg6, 2gga Gene: aroA (AGRSC) Activity: 3-phosphoshikimate 1-carboxyvinyltransferase, with EC number 2.5.1.19 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The engineering of transgenic crops resistant to the broad-spectrum herbicide glyphosate has greatly improved agricultural efficiency worldwide. Glyphosate-based herbicides, such as Roundup, target the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, the functionality of which is absolutely required for the survival of plants. Roundup Ready plants carry the gene coding for a glyphosate-insensitive form of this enzyme, obtained from Agrobacterium sp. strain CP4. Once incorporated into the plant genome, the gene product, CP4 EPSP synthase, confers crop resistance to glyphosate. Although widely used, the molecular basis for this glyphosate-resistance has remained obscure. We generated a synthetic gene coding for CP4 EPSP synthase and characterized the enzyme using kinetics and crystallography. The CP4 enzyme has unexpected kinetic and structural properties that render it unique among the known EPSP synthases. Glyphosate binds to the CP4 EPSP synthase in a condensed, noninhibitory conformation. Glyphosate sensitivity can be restored through a single-site mutation in the active site (Ala-100-Gly), allowing glyphosate to bind in its extended, inhibitory conformation. Molecular basis for the herbicide resistance of Roundup Ready crops.,Funke T, Han H, Healy-Fried ML, Fischer M, Schonbrunn E Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5. Epub 2006 Aug 17. PMID:16916934 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also EPSP synthase

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