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==Alteration of sequence specificity of the type II restriction endonuclease HINCII through an indirect readout mechanism==
==Alteration of sequence specificity of the type II restriction endonuclease HINCII through an indirect readout mechanism==
<StructureSection load='2gig' size='340' side='right' caption='[[2gig]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
<StructureSection load='2gig' size='340' side='right'caption='[[2gig]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2gig]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GIG FirstGlance]. <br>
<table><tr><td colspan='2'>[[2gig]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIG FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2aud|2aud]], [[1xhv|1xhv]], [[1hxu|1hxu]], [[1tw8|1tw8]], [[1kc6|1kc6]], [[2gie|2gie]], [[2gih|2gih]], [[2gii|2gii]], [[2gij|2gij]], [[1tx3|1tx3]]</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2aud|2aud]], [[1xhv|1xhv]], [[1hxu|1hxu]], [[1tw8|1tw8]], [[1kc6|1kc6]], [[2gie|2gie]], [[2gih|2gih]], [[2gii|2gii]], [[2gij|2gij]], [[1tx3|1tx3]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hincIIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hincIIR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gig OCA], [http://pdbe.org/2gig PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gig RCSB], [http://www.ebi.ac.uk/pdbsum/2gig PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gig ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gig OCA], [https://pdbe.org/2gig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gig RCSB], [https://www.ebi.ac.uk/pdbsum/2gig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gig ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/T2D2_HAEIN T2D2_HAEIN]] Recognizes the double-stranded sequence GTYRAC and cleaves after Y-3.  
[[https://www.uniprot.org/uniprot/T2D2_HAEIN T2D2_HAEIN]] Recognizes the double-stranded sequence GTYRAC and cleaves after Y-3.  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</div>
</div>
<div class="pdbe-citations 2gig" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 2gig" style="background-color:#fffaf0;"></div>
==See Also==
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Bacterium influenzae lehmann and neumann 1896]]
[[Category: Bacterium influenzae lehmann and neumann 1896]]
[[Category: Large Structures]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Bitinaite, J]]
[[Category: Bitinaite, J]]
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