2ddb

Crystal structure of pseudecin from Pseudechis porphyriacus

spinqualitylabelsall models 2ddb, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2ddb is a 4 chain structure with sequence from Psepo. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FMT, GOL, NA Related: 2dda Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [CRVP_PSEPO] Blocks olfactory (CNGA2) and retinal (CNGA1) CNG channel currents. Is really less potent that Pseudechetoxin. Does not affect neither depolarization- nor caffeine-induced contraction of smooth muscle. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Cyclic nucleotide-gated (CNG) ion channels play pivotal roles in sensory transduction by retinal photoreceptors and olfactory neurons. The elapid snake toxins pseudechetoxin (PsTx) and pseudecin (Pdc) are the only known protein blockers of CNG channels. These toxins belong to a cysteine-rich secretory protein (CRISP) family containing an N-terminal pathogenesis-related proteins of group 1 (PR-1) domain and a C-terminal cysteine-rich domain (CRD). PsTx and Pdc are highly homologous proteins, but their blocking affinities on CNG channels are different: PsTx blocks both the olfactory and retinal channels with approximately 15-30-fold higher affinity than Pdc. To gain further insights into their structure and function, the crystal structures of PsTx, Pdc and Zn2+-bound Pdc were determined. The structures revealed that most of the amino-acid-residue differences between PsTx and Pdc are located around the concave surface formed between the PR-1 domain and the CRD, suggesting that the concave surface is functionally important for CNG-channel binding and inhibition. A structural comparison in the presence and absence of Zn2+ ion demonstrated that the concave surface can open and close owing to movement of the CRD upon Zn2+ binding. The data suggest that PsTx and Pdc occlude the pore entrance and that the dynamic motion of the concave surface facilitates interaction with the CNG channels. Structures of pseudechetoxin and pseudecin, two snake-venom cysteine-rich secretory proteins that target cyclic nucleotide-gated ion channels: implications for movement of the C-terminal cysteine-rich domain.,Suzuki N, Yamazaki Y, Brown RL, Fujimoto Z, Morita T, Mizuno H Acta Crystallogr D Biol Crystallogr. 2008 Oct;64(Pt 10):1034-42. Epub 2008, Sep 19. PMID:18931410 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Pseudechetoxin

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