1ef4: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1ef4.gif|left|200px]] | [[Image:1ef4.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1ef4", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
| | --> | ||
| | {{STRUCTURE_1ef4| PDB=1ef4 | SCENE= }} | ||
}} | |||
'''SOLUTION STRUCTURE OF THE ESSENTIAL RNA POLYMERASE SUBUNIT RPB10 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM''' | '''SOLUTION STRUCTURE OF THE ESSENTIAL RNA POLYMERASE SUBUNIT RPB10 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM''' | ||
| Line 31: | Line 28: | ||
[[Category: Mcintosh, L P.]] | [[Category: Mcintosh, L P.]] | ||
[[Category: NESG, Northeast Structural Genomics Consortium.]] | [[Category: NESG, Northeast Structural Genomics Consortium.]] | ||
[[Category: | [[Category: Nesg]] | ||
[[Category: | [[Category: Northeast structural genomics consortium]] | ||
[[Category: | [[Category: Protein structure initiative]] | ||
[[Category: | [[Category: Psi]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: | [[Category: Three helix bundle]] | ||
[[Category: | [[Category: Zinc binding]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:01:18 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 12:01, 2 May 2008
SOLUTION STRUCTURE OF THE ESSENTIAL RNA POLYMERASE SUBUNIT RPB10 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM
Overview
The RNA polymerase subunit RPB10 displays a high level of conservation across archaea and eukarya and is required for cell viability in yeast. Structure determination of this RNA polymerase subunit from Methanobacterium thermoautotrophicum reveals a topology, which we term a zinc-bundle, consisting of three alpha-helices stabilized by a zinc ion. The metal ion is bound within an atypical CX(2)CX(n)CC sequence motif and serves to bridge an N-terminal loop with helix 3. This represents an example of two adjacent zinc-binding Cys residues within an alpha-helix conformation. Conserved surface features of RPB10 include discrete regions of neutral, acidic, and basic residues, the latter being located around the zinc-binding site. One or more of these regions may contribute to the role of this subunit as a scaffold protein within the polymerase holoenzyme.
About this Structure
1EF4 is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.
Reference
Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum., Mackereth CD, Arrowsmith CH, Edwards AM, McIntosh LP, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6316-21. PMID:10841539 Page seeded by OCA on Fri May 2 15:01:18 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- DNA-directed RNA polymerase
- Methanothermobacter thermautotrophicus
- Single protein
- Arrowsmith, C H.
- Edwards, A M.
- Mackereth, C D.
- Mcintosh, L P.
- NESG, Northeast Structural Genomics Consortium.
- Nesg
- Northeast structural genomics consortium
- Protein structure initiative
- Psi
- Structural genomic
- Three helix bundle
- Zinc binding
