2m3g

Structure of Anabaena Sensory Rhodopsin Determined by Solid State NMR Spectroscopy

spinqualitylabelsall models 2m3g, 10 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2m3g is a 3 chain structure with sequence from Anabaena 7120. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: RET Gene: alr3165 (Anabaena 7120) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Determination of structure of integral membrane proteins, especially in their native environment, is a formidable challenge in structural biology. Here we demonstrate that magic angle spinning solid-state NMR spectroscopy can be used to determine structures of membrane proteins reconstituted in synthetic lipids, an environment similar to the natural membrane. We combined a large number of experimentally determined interatomic distances and local torsional restraints to solve the structure of an oligomeric membrane protein of common seven-helical fold, Anabaena sensory rhodopsin (ASR). We determined the atomic resolution detail of the oligomerization interface of the ASR trimer, and the arrangement of helices, side chains and the retinal cofactor in the monomer. Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein.,Wang S, Munro RA, Shi L, Kawamura I, Okitsu T, Wada A, Kim SY, Jung KH, Brown LS, Ladizhansky V Nat Methods. 2013 Sep 8. doi: 10.1038/nmeth.2635. PMID:24013819 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Bacteriorhodopsin 3D structures Rhodopsin 3D structures

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