2nar

Solution structure of AVR3a_60-147 from Phytophthora infestans

spinqualitylabelsall models 2nar, 10 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2nar is a 1 chain structure with sequence from Botrytis infestans. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: Avr3a, PI35.0050, PI49.0530, putative (Botrytis infestans) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed When plant-pathogenic oomycetes infect their hosts, they employ a large arsenal of effector proteins to establish a successful infection. Some effector proteins are secreted and are destined to be translocated and function inside host cells. The largest group of translocated proteins from oomycetes is the RxLR effectors, defined by their conserved N-terminal Arg-Xaa-Leu-Arg (RxLR) motif. However, the precise role of this motif in the host cell translocation process is unclear. Here, detailed biochemical studies of the RxLR effector AVR3a from the potato pathogen Phytophthora infestans are presented. Mass spectrometric analysis revealed that the RxLR sequence of native AVR3a is cleaved off prior to secretion by the pathogen and the N terminus of the mature effector was found likely to be acetylated. High-resolution NMR structure analysis of AVR3a indicates that the RxLR motif is well accessible to potential processing enzymes. Processing and modification of AVR3a is to some extent similar to events occurring with the export element (PEXEL) found in malaria effector proteins from Plasmodium falciparum These findings imply a role for the RxLR motif in the secretion of AVR3a by the pathogen, rather than a direct role in the host cell entry process itself. The RxLR Motif of the Host Targeting Effector AVR3a of Phytophthora infestans Is Cleaved before Secretion.,Wawra S, Trusch F, Matena A, Apostolakis K, Linne U, Zhukov I, Stanek J, Kozminski W, Davidson I, Secombes CJ, Bayer P, van West P Plant Cell. 2017 Jun;29(6):1184-1195. doi: 10.1105/tpc.16.00552. Epub 2017 May, 18. PMID:28522546 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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