2qj5

PYP ultra-high resolution of a bacterial photoreceptor

spinqualitylabelsall models 2qj5, resolution 1.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2qj5 is a 1 chain structure with sequence from Halorhodospira halophila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: HC4 Related: 2qj7 Gene: pyp (Halorhodospira halophila) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Protein structural fluctuations occur over a wide spatial scale, ranging from minute, picometer-scale displacements, to large, interdomain motions and partial unfolding. While large-scale protein structural changes and their effects on protein function have been the focus of much recent attention, small-scale fluctuations have been less well studied, and are generally assumed to have proportionally smaller effects. Here we use the bacterial photoreceptor photoactive yellow protein (PYP) to test if subtle structural changes do, indeed, imply equally subtle functional effects. We flash froze crystals of PYP to trap the protein's conformational ensemble, and probed the molecules in this ensemble for their ability to facilitate PYP's biological function (i.e., light-driven isomerization of its chromophore). Our results indicate that the apparently homogeneous structural state observed in a 0.82 A crystal structure in fact comprises an ensemble of conformational states, in which subpopulations with nearly identical structures display dramatically different functional properties. Picometer-scale conformational heterogeneity separates functional from nonfunctional states of a photoreceptor protein.,Coureux PD, Fan ZP, Stojanoff V, Genick UK Structure. 2008 Jun;16(6):863-72. PMID:18547519 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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